The perfect stage and SSR analysis of Magnaporthe grisea in the Yanxi Blast Nursery, Hunan Province
One hundred and twenty-four Magnaporthe grisea isolates collected in four years from the Yanxi Blast Nursery, Hunan Province were tested for mating type with four hermaphroditic fertile isolates MAT1. 1 (KA3 and TH12) and MAT1. 2 (Guy11 and TH16) and the genetic diversity of 105 isolates were analyzed by using microsatellite markers(SSR). Among the 124 isolates, 28 belonged to MAT1. 1, 39 to MAT1. 2 and the remaining 57 to sterile ones, taking 22. 58%, 31. 45% and 45. 97% for each group, respectively. The 105 isolates could be divided into six lineages at 0. 67 similarity level based on the amplicons with seven pairs of SSR primers.
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| Main Authors: | , , , |
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| Format: | article biblioteca |
| Language: | chi |
| Subjects: | H20 - Maladies des plantes, Magnaporthe grisea, variation génétique, microsatellite, marqueur génétique, http://aims.fao.org/aos/agrovoc/c_37090, http://aims.fao.org/aos/agrovoc/c_15975, http://aims.fao.org/aos/agrovoc/c_36574, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_1556, |
| Online Access: | http://agritrop.cirad.fr/523100/ |
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| Summary: | One hundred and twenty-four Magnaporthe grisea isolates collected in four years from the Yanxi Blast Nursery, Hunan Province were tested for mating type with four hermaphroditic fertile isolates MAT1. 1 (KA3 and TH12) and MAT1. 2 (Guy11 and TH16) and the genetic diversity of 105 isolates were analyzed by using microsatellite markers(SSR). Among the 124 isolates, 28 belonged to MAT1. 1, 39 to MAT1. 2 and the remaining 57 to sterile ones, taking 22. 58%, 31. 45% and 45. 97% for each group, respectively. The 105 isolates could be divided into six lineages at 0. 67 similarity level based on the amplicons with seven pairs of SSR primers. |
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