Effect of water content and temperature on Carica papaya lipase catalyzed esterification and transesterification reactions

Temperature and water activity (a.) of the reaction medium are two factors that govern enzyme reactions. We studied the influence of these two parameters on the esterification and transesterification activity of Carica papaya lipase in water and solvent free reactions. It was found that over the course of reaction the catalytic activity of C. papaya lipase was dependent on these factors. The best lipase activity for both reactions was at a temperature of 55°C and water activity of 0.22, which corresponds to 2 g of water per 100 g of C. papaya latex.

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Bibliographic Details
Main Authors: Turon, Fabrice, Caro, Yanis, Villeneuve, Pierre, Pina, Michel, Graille, Jean
Format: article biblioteca
Language:eng
Subjects:Q02 - Traitement et conservation des produits alimentaires, Carica papaya, papaye, estérase, estérification, teneur en eau, température, Activité catalytique, Activité de l'eau, http://aims.fao.org/aos/agrovoc/c_1323, http://aims.fao.org/aos/agrovoc/c_13087, http://aims.fao.org/aos/agrovoc/c_2670, http://aims.fao.org/aos/agrovoc/c_28361, http://aims.fao.org/aos/agrovoc/c_4886, http://aims.fao.org/aos/agrovoc/c_7657, http://aims.fao.org/aos/agrovoc/c_1382, http://aims.fao.org/aos/agrovoc/c_24929,
Online Access:http://agritrop.cirad.fr/519451/
http://agritrop.cirad.fr/519451/1/document_519451.pdf
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Summary:Temperature and water activity (a.) of the reaction medium are two factors that govern enzyme reactions. We studied the influence of these two parameters on the esterification and transesterification activity of Carica papaya lipase in water and solvent free reactions. It was found that over the course of reaction the catalytic activity of C. papaya lipase was dependent on these factors. The best lipase activity for both reactions was at a temperature of 55°C and water activity of 0.22, which corresponds to 2 g of water per 100 g of C. papaya latex.