Genetic diversity among wild sugarcane germplasm from Laos revealed with markers
A group of 35 wild Saccharum complex clones, collected in Laos (ECL), was studied for its nuclear genetic diversity by RFLP revealed by 10 dispersed low copy probes. Representatives of the main germplasm diversity, regularly used in introgression, were also included. A preliminary characterisation of the new clones was performed by Erianthus specific primers. A set of the 11 ECL clones most outstanding for their disease resistance and vigor and basic germplasm members were also characterised by 9 cytoplasmic probes. Nuclear diversity evidenced that ECL clones represented an independent genetic pool consisting at least of 3 different groups. A group of bands was exclusively exhibited by ECL clones. The occurrence of genetic recombination among them was suggested by the presence of the new bands in 2 or 3 clonal groups. Erianthus specific amplification was positive for 7 ECL clones from different nuclear groups. Dendrogram analysis based on cytoplasmic diversity divided genotypes into 2 major cytoplasmic types comprising 5 groups. The ECL clones separated in 2 distinct groups: one located in the same cytoplasmic type as Erianthus clones; the other one, in an intermediate position, closer to Saccharum species. Basic clones groups were consistent with their taxonomic classification. The diversity revealed by nuclear single copy/nuclear repetitive/cytoplasmic patterns of the ECL germplasm contrasts with what is usually observed and points to an active population evolution. Further studies needed to confirmed this hypothesis are discussed.
| Main Authors: | , , , , , |
|---|---|
| Format: | article biblioteca |
| Language: | eng |
| Subjects: | F30 - Génétique et amélioration des plantes, Saccharum, clone, variation génétique, RFLP, germoplasme, Erianthus, pool de gènes, marqueur génétique, http://aims.fao.org/aos/agrovoc/c_6725, http://aims.fao.org/aos/agrovoc/c_1678, http://aims.fao.org/aos/agrovoc/c_15975, http://aims.fao.org/aos/agrovoc/c_34255, http://aims.fao.org/aos/agrovoc/c_3249, http://aims.fao.org/aos/agrovoc/c_33698, http://aims.fao.org/aos/agrovoc/c_3212, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_12076, |
| Online Access: | http://agritrop.cirad.fr/519447/ http://agritrop.cirad.fr/519447/1/519447.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | A group of 35 wild Saccharum complex clones, collected in Laos (ECL), was studied for its nuclear genetic diversity by RFLP revealed by 10 dispersed low copy probes. Representatives of the main germplasm diversity, regularly used in introgression, were also included. A preliminary characterisation of the new clones was performed by Erianthus specific primers. A set of the 11 ECL clones most outstanding for their disease resistance and vigor and basic germplasm members were also characterised by 9 cytoplasmic probes. Nuclear diversity evidenced that ECL clones represented an independent genetic pool consisting at least of 3 different groups. A group of bands was exclusively exhibited by ECL clones. The occurrence of genetic recombination among them was suggested by the presence of the new bands in 2 or 3 clonal groups. Erianthus specific amplification was positive for 7 ECL clones from different nuclear groups. Dendrogram analysis based on cytoplasmic diversity divided genotypes into 2 major cytoplasmic types comprising 5 groups. The ECL clones separated in 2 distinct groups: one located in the same cytoplasmic type as Erianthus clones; the other one, in an intermediate position, closer to Saccharum species. Basic clones groups were consistent with their taxonomic classification. The diversity revealed by nuclear single copy/nuclear repetitive/cytoplasmic patterns of the ECL germplasm contrasts with what is usually observed and points to an active population evolution. Further studies needed to confirmed this hypothesis are discussed. |
|---|