Analysis of latex expressed sequence tags (ESTs) in Hevea brasiliensis
Natural rubber from Hevea brasiliensis latex consists of a high molecular weight polymer composed of isopentenyl diphosphate (IPP) molecules. Latex is the cytoplasmic fluid contained in the vessel tubes or laticifers which are located in the bark of the Hevea tree. As cytoplasm, latex would be expected to contain general cellular processes carried out by eukaryotic cells and more specifically, rubber biosynthesis. In this project, we are interested in building a profile of genes expressed in latex. These expressed genes or Expressed Sequence Tags (ESTs) are obtained by sequencing random cDNA clones which represent the genes transcribed in latex. Our objective is to generate a pool of Hevea ESTs which would serve as a molecular genetics resource to facilitate various aspects of rubber research. Furthermore, an EST library will also create the way for microarray-based studies of Hevea gene expression. In this study, we report the current status of EST sequencing of clones from a latex cDNA library.
| Main Authors: | , , , , |
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| Format: | conference_item biblioteca |
| Language: | eng |
| Published: |
CIRAD
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| Subjects: | F30 - Génétique et amélioration des plantes, |
| Online Access: | http://agritrop.cirad.fr/513737/ http://agritrop.cirad.fr/513737/1/ID513737.pdf |
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| Summary: | Natural rubber from Hevea brasiliensis latex consists of a high molecular weight polymer composed of isopentenyl diphosphate (IPP) molecules. Latex is the cytoplasmic fluid contained in the vessel tubes or laticifers which are located in the bark of the Hevea tree. As cytoplasm, latex would be expected to contain general cellular processes carried out by eukaryotic cells and more specifically, rubber biosynthesis. In this project, we are interested in building a profile of genes expressed in latex. These expressed genes or Expressed Sequence Tags (ESTs) are obtained by sequencing random cDNA clones which represent the genes transcribed in latex. Our objective is to generate a pool of Hevea ESTs which would serve as a molecular genetics resource to facilitate various aspects of rubber research. Furthermore, an EST library will also create the way for microarray-based studies of Hevea gene expression. In this study, we report the current status of EST sequencing of clones from a latex cDNA library. |
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