Manual pollination techniques and verification of incompatibility : Implementation of selection trials and data collection
Pollination in cocoa must be controlled in order to obtain the expected result. In the past, errors in results from hand pollination were identified by using isozymes and more recently, DNA markers. Even with strict control of hand pollination, a small percentage of unwanted fertilizations can still take place. Possible sources of errors are: - Incomplete isolation of flowers (crawling insects may enter the pollination tubes); - Emasculation of flowers before pollination: this unnecessary manipulation may induce selfing and contamination of the pollination tweezers; - Other manipulations of the flowers can contaminate the pistil with self-pollen; - Successive use of different types of pollen, without cleaning the tweezers with alcohol between manipulations. A description is given of a manual pollination technique, including self and crosspollination, the use of mentor pollen to overcome self-incompatibility, and comparison of this "standard" pollination procedure with locally applied methods.
| Main Author: | |
|---|---|
| Format: | conference_item biblioteca |
| Language: | eng |
| Published: |
IPGRI
|
| Subjects: | F63 - Physiologie végétale - Reproduction, Theobroma cacao, pollinisation artificielle, pollinisation croisée, incompatibilité pollinique, méthode, http://aims.fao.org/aos/agrovoc/c_7713, http://aims.fao.org/aos/agrovoc/c_36531, http://aims.fao.org/aos/agrovoc/c_1973, http://aims.fao.org/aos/agrovoc/c_6071, http://aims.fao.org/aos/agrovoc/c_4788, |
| Online Access: | http://agritrop.cirad.fr/475698/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Pollination in cocoa must be controlled in order to obtain the expected result. In the past, errors in results from hand pollination were identified by using isozymes and more recently, DNA markers. Even with strict control of hand pollination, a small percentage of unwanted fertilizations can still take place. Possible sources of errors are: - Incomplete isolation of flowers (crawling insects may enter the pollination tubes); - Emasculation of flowers before pollination: this unnecessary manipulation may induce selfing and contamination of the pollination tweezers; - Other manipulations of the flowers can contaminate the pistil with self-pollen; - Successive use of different types of pollen, without cleaning the tweezers with alcohol between manipulations. A description is given of a manual pollination technique, including self and crosspollination, the use of mentor pollen to overcome self-incompatibility, and comparison of this "standard" pollination procedure with locally applied methods. |
|---|