Oil palm (Elaeis guineensis Jacq.) regeneration from embryogenic suspension culture
Oil palm, vegetative propagation on solid medium is performed on a large scale for several years. In order to improve efficiency of the process, investigations on liquid medium were carried out. Embryogenic calli produced from leaf explants of adult oil palm were placed in Murashige and Skoog's modified liquid medium containing 2,4-dichlorophenoxyacetic acid (2,4-D), cytokinins and activated charcoal. White globular aggregates were released in the medium during the first subculture and proliferated. After removal of the callus tissue, a suspension was established. It was made of meristematic clumps, as confirmed by histological examinations. Culture conditions were studied to improve the proliferation rate. The liquid culture has been maintained for over 18 months without any loss of embryogenic capacity. Following transfer to an hormone-free liquid medium, proliferation stopped and differentiation of clumps towards embryos has been observed. After plating on the some medium, the maturation of embryos was achieved and regenerated plants were successfully transferred to soil.
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Subjects: | F02 - Multiplication végétative des plantes, Elaeis guineensis, multiplication végétative, Embryogénèse somatique, milieu de culture, http://aims.fao.org/aos/agrovoc/c_2509, http://aims.fao.org/aos/agrovoc/c_8177, http://aims.fao.org/aos/agrovoc/c_36911, http://aims.fao.org/aos/agrovoc/c_10204, |
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dig-cirad-fr-4012572024-01-28T01:14:55Z http://agritrop.cirad.fr/401257/ http://agritrop.cirad.fr/401257/ Oil palm (Elaeis guineensis Jacq.) regeneration from embryogenic suspension culture. De Touchet B., Duval Yves, Pannetier Catherine. 1990. In : Abstracts VIIth international congress on plant tissue and cell culture = [Résumés du 7ème congrès international sur les cultures de tissus et de cellules des plantes]. IAPTC. Amsterdam : IAPTC, 1 p. International Congress on Plant Tissue and Cell Culture. 7, Amsterdam, Pays-Bas, 24 Juin 1990/29 Juin 1990. Oil palm (Elaeis guineensis Jacq.) regeneration from embryogenic suspension culture De Touchet, B. Duval, Yves Pannetier, Catherine eng 1990 IAPTC Abstracts VIIth international congress on plant tissue and cell culture = [Résumés du 7ème congrès international sur les cultures de tissus et de cellules des plantes] F02 - Multiplication végétative des plantes Elaeis guineensis multiplication végétative Embryogénèse somatique milieu de culture http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_8177 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_10204 Oil palm, vegetative propagation on solid medium is performed on a large scale for several years. In order to improve efficiency of the process, investigations on liquid medium were carried out. Embryogenic calli produced from leaf explants of adult oil palm were placed in Murashige and Skoog's modified liquid medium containing 2,4-dichlorophenoxyacetic acid (2,4-D), cytokinins and activated charcoal. White globular aggregates were released in the medium during the first subculture and proliferated. After removal of the callus tissue, a suspension was established. It was made of meristematic clumps, as confirmed by histological examinations. Culture conditions were studied to improve the proliferation rate. The liquid culture has been maintained for over 18 months without any loss of embryogenic capacity. Following transfer to an hormone-free liquid medium, proliferation stopped and differentiation of clumps towards embryos has been observed. After plating on the some medium, the maturation of embryos was achieved and regenerated plants were successfully transferred to soil. conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/closedAccess http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=98144 |
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F02 - Multiplication végétative des plantes Elaeis guineensis multiplication végétative Embryogénèse somatique milieu de culture http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_8177 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_10204 F02 - Multiplication végétative des plantes Elaeis guineensis multiplication végétative Embryogénèse somatique milieu de culture http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_8177 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_10204 |
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F02 - Multiplication végétative des plantes Elaeis guineensis multiplication végétative Embryogénèse somatique milieu de culture http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_8177 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_10204 F02 - Multiplication végétative des plantes Elaeis guineensis multiplication végétative Embryogénèse somatique milieu de culture http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_8177 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_10204 De Touchet, B. Duval, Yves Pannetier, Catherine Oil palm (Elaeis guineensis Jacq.) regeneration from embryogenic suspension culture |
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Oil palm, vegetative propagation on solid medium is performed on a large scale for several years. In order to improve efficiency of the process, investigations on liquid medium were carried out. Embryogenic calli produced from leaf explants of adult oil palm were placed in Murashige and Skoog's modified liquid medium containing 2,4-dichlorophenoxyacetic acid (2,4-D), cytokinins and activated charcoal. White globular aggregates were released in the medium during the first subculture and proliferated. After removal of the callus tissue, a suspension was established. It was made of meristematic clumps, as confirmed by histological examinations. Culture conditions were studied to improve the proliferation rate. The liquid culture has been maintained for over 18 months without any loss of embryogenic capacity. Following transfer to an hormone-free liquid medium, proliferation stopped and differentiation of clumps towards embryos has been observed. After plating on the some medium, the maturation of embryos was achieved and regenerated plants were successfully transferred to soil. |
format |
conference_item |
topic_facet |
F02 - Multiplication végétative des plantes Elaeis guineensis multiplication végétative Embryogénèse somatique milieu de culture http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_8177 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_10204 |
author |
De Touchet, B. Duval, Yves Pannetier, Catherine |
author_facet |
De Touchet, B. Duval, Yves Pannetier, Catherine |
author_sort |
De Touchet, B. |
title |
Oil palm (Elaeis guineensis Jacq.) regeneration from embryogenic suspension culture |
title_short |
Oil palm (Elaeis guineensis Jacq.) regeneration from embryogenic suspension culture |
title_full |
Oil palm (Elaeis guineensis Jacq.) regeneration from embryogenic suspension culture |
title_fullStr |
Oil palm (Elaeis guineensis Jacq.) regeneration from embryogenic suspension culture |
title_full_unstemmed |
Oil palm (Elaeis guineensis Jacq.) regeneration from embryogenic suspension culture |
title_sort |
oil palm (elaeis guineensis jacq.) regeneration from embryogenic suspension culture |
publisher |
IAPTC |
url |
http://agritrop.cirad.fr/401257/ |
work_keys_str_mv |
AT detouchetb oilpalmelaeisguineensisjacqregenerationfromembryogenicsuspensionculture AT duvalyves oilpalmelaeisguineensisjacqregenerationfromembryogenicsuspensionculture AT pannetiercatherine oilpalmelaeisguineensisjacqregenerationfromembryogenicsuspensionculture |
_version_ |
1792492060228452352 |