Oil palm (Elaeis guineensis Jacq.) regeneration from embryogenic suspension culture
Oil palm, vegetative propagation on solid medium is performed on a large scale for several years. In order to improve efficiency of the process, investigations on liquid medium were carried out. Embryogenic calli produced from leaf explants of adult oil palm were placed in Murashige and Skoog's modified liquid medium containing 2,4-dichlorophenoxyacetic acid (2,4-D), cytokinins and activated charcoal. White globular aggregates were released in the medium during the first subculture and proliferated. After removal of the callus tissue, a suspension was established. It was made of meristematic clumps, as confirmed by histological examinations. Culture conditions were studied to improve the proliferation rate. The liquid culture has been maintained for over 18 months without any loss of embryogenic capacity. Following transfer to an hormone-free liquid medium, proliferation stopped and differentiation of clumps towards embryos has been observed. After plating on the some medium, the maturation of embryos was achieved and regenerated plants were successfully transferred to soil.
| Main Authors: | , , |
|---|---|
| Format: | conference_item biblioteca |
| Language: | eng |
| Published: |
IAPTC
|
| Subjects: | F02 - Multiplication végétative des plantes, Elaeis guineensis, multiplication végétative, Embryogénèse somatique, milieu de culture, http://aims.fao.org/aos/agrovoc/c_2509, http://aims.fao.org/aos/agrovoc/c_8177, http://aims.fao.org/aos/agrovoc/c_36911, http://aims.fao.org/aos/agrovoc/c_10204, |
| Online Access: | http://agritrop.cirad.fr/401257/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Oil palm, vegetative propagation on solid medium is performed on a large scale for several years. In order to improve efficiency of the process, investigations on liquid medium were carried out. Embryogenic calli produced from leaf explants of adult oil palm were placed in Murashige and Skoog's modified liquid medium containing 2,4-dichlorophenoxyacetic acid (2,4-D), cytokinins and activated charcoal. White globular aggregates were released in the medium during the first subculture and proliferated. After removal of the callus tissue, a suspension was established. It was made of meristematic clumps, as confirmed by histological examinations. Culture conditions were studied to improve the proliferation rate. The liquid culture has been maintained for over 18 months without any loss of embryogenic capacity. Following transfer to an hormone-free liquid medium, proliferation stopped and differentiation of clumps towards embryos has been observed. After plating on the some medium, the maturation of embryos was achieved and regenerated plants were successfully transferred to soil. |
|---|