Découverte du tomato yellow leaf curl virus (TYLCV) à la Réunion
In September 1997, leaf curling and yellowing were observed on tomato plants on a farm on the south coast of La Reunion, a French island in the Mascarenes archipelago. Based on the similarities of the symptoms caused by geminiviruses on tomato, the diagnosis was oriented towards the detection of a geminivirus. Using degenerate primers designed to amplify a region of the A component of most of the begomoviruses (formerly sub-group 111 geminiviruses), a 500 base pair PCR product was obtained with a diseased plant, but not with a healthy looking one. Based on sequence similarities, it was shown that the detected geminivirus was an isolate of the tomato yellow leaf curl virus from Israel (TYLCV-IL).
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| Main Authors: | , , |
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| Format: | book_section biblioteca |
| Language: | fre |
| Published: |
CIRAD
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| Subjects: | H20 - Maladies des plantes, géminivirus enroulement jaune tomat, symptome, carte génétique, http://aims.fao.org/aos/agrovoc/c_32611, http://aims.fao.org/aos/agrovoc/c_7566, http://aims.fao.org/aos/agrovoc/c_24002, http://aims.fao.org/aos/agrovoc/c_6543, http://aims.fao.org/aos/agrovoc/c_3081, |
| Online Access: | http://agritrop.cirad.fr/392285/ |
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| Summary: | In September 1997, leaf curling and yellowing were observed on tomato plants on a farm on the south coast of La Reunion, a French island in the Mascarenes archipelago. Based on the similarities of the symptoms caused by geminiviruses on tomato, the diagnosis was oriented towards the detection of a geminivirus. Using degenerate primers designed to amplify a region of the A component of most of the begomoviruses (formerly sub-group 111 geminiviruses), a 500 base pair PCR product was obtained with a diseased plant, but not with a healthy looking one. Based on sequence similarities, it was shown that the detected geminivirus was an isolate of the tomato yellow leaf curl virus from Israel (TYLCV-IL). |
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