Effect of technique and darkness on the success of meristem micrografting of Picea abies
The possibility of micrografting in vitro shoot meristems of #Picea abies# were investigated on a 18 year-old Norway spruce clone. The rates of success were shown to be greatly influenced by the grafting technique used and by light, with a positive effect of a 2 to 3 week darkness period applied to the stocks just after they had been grafted. Average scores of more than 50% of success were obtained. However, substantial variability in terms of shoot expansion among the grafted plants existed in vitro, as well as after transfer to ex-vitro conditions.
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Format: | article biblioteca |
Language: | eng |
Subjects: | F02 - Multiplication végétative des plantes, K10 - Production forestière, |
Online Access: | http://agritrop.cirad.fr/391119/ http://agritrop.cirad.fr/391119/1/SilGen1994.pdf |
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Summary: | The possibility of micrografting in vitro shoot meristems of #Picea abies# were investigated on a 18 year-old Norway spruce clone. The rates of success were shown to be greatly influenced by the grafting technique used and by light, with a positive effect of a 2 to 3 week darkness period applied to the stocks just after they had been grafted. Average scores of more than 50% of success were obtained. However, substantial variability in terms of shoot expansion among the grafted plants existed in vitro, as well as after transfer to ex-vitro conditions. |
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