Effect of technique and darkness on the success of meristem micrografting of Picea abies

The possibility of micrografting in vitro shoot meristems of #Picea abies# were investigated on a 18 year-old Norway spruce clone. The rates of success were shown to be greatly influenced by the grafting technique used and by light, with a positive effect of a 2 to 3 week darkness period applied to the stocks just after they had been grafted. Average scores of more than 50% of success were obtained. However, substantial variability in terms of shoot expansion among the grafted plants existed in vitro, as well as after transfer to ex-vitro conditions.

Saved in:
Bibliographic Details
Main Author: Monteuuis, Olivier
Format: article biblioteca
Language:eng
Subjects:F02 - Multiplication végétative des plantes, K10 - Production forestière,
Online Access:http://agritrop.cirad.fr/391119/
http://agritrop.cirad.fr/391119/1/SilGen1994.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The possibility of micrografting in vitro shoot meristems of #Picea abies# were investigated on a 18 year-old Norway spruce clone. The rates of success were shown to be greatly influenced by the grafting technique used and by light, with a positive effect of a 2 to 3 week darkness period applied to the stocks just after they had been grafted. Average scores of more than 50% of success were obtained. However, substantial variability in terms of shoot expansion among the grafted plants existed in vitro, as well as after transfer to ex-vitro conditions.