A genetic linkage map of Theobroma cacao L. based on isozymes, RFLPs, and RAPDs markers
A linkage map of cacao genome have been constructed using isoenzymes, RFLPOs, and RAPDs markers. A genomic library and cDNA library were constructed from germinated seeds of an Amelonado clone. The screening of probes was made on 5 genotypes, using 5 restriction enzymes. Among 587 tested probes, 52% of cDNA and 44% of genomic probes revealed polymorphic patterns. A population of 100 individuals derived from a cross between two heterozygous genotypes has been used. Segregation analyses were performed with JoinMap programm. The map comprised 193 loci: 5 isoenzymes, 101 cDNA/RFLPs, 4 loci from genes of known fonction, 55 genomic DNA/RFLPs and 28 RAPDs. Ten linkage groups, which putatively correspond to the 10 gametic chromosomes of cacao, have been identified. The map covers a total length of 759 cM with a 3,9 cM average distance between two markers. A small fraction (9%) of the markers deviated significantly from the expected mendelian ratios
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Subjects: | F30 - Génétique et amélioration des plantes, Theobroma cacao, adn, carte génétique, RAPD, RFLP, isoenzyme, liaison génétique, http://aims.fao.org/aos/agrovoc/c_7713, http://aims.fao.org/aos/agrovoc/c_2347, http://aims.fao.org/aos/agrovoc/c_24002, http://aims.fao.org/aos/agrovoc/c_36341, http://aims.fao.org/aos/agrovoc/c_34255, http://aims.fao.org/aos/agrovoc/c_15987, http://aims.fao.org/aos/agrovoc/c_11179da9, |
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dig-cirad-fr-3897122024-01-27T23:01:56Z http://agritrop.cirad.fr/389712/ http://agritrop.cirad.fr/389712/ A genetic linkage map of Theobroma cacao L. based on isozymes, RFLPs, and RAPDs markers. Lanaud Claire, Risterucci Ange-Marie, N'Goran Jeanne A.K., Clément Didier, Flament Marie-Henriette, Laurent V.. 1995. In : Plant genome III = [Génome des plantes III]. Scherago International. s.l. : s.n., 56. International Conference on the Status of Plant Genome Research. 3, San Diego, États-Unis, 15 Janvier 1995/19 Janvier 1995. A genetic linkage map of Theobroma cacao L. based on isozymes, RFLPs, and RAPDs markers Lanaud, Claire Risterucci, Ange-Marie N'Goran, Jeanne A.K. Clément, Didier Flament, Marie-Henriette Laurent, V. eng 1995 s.n. Plant genome III = [Génome des plantes III] F30 - Génétique et amélioration des plantes Theobroma cacao adn carte génétique RAPD RFLP isoenzyme liaison génétique http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_36341 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_15987 http://aims.fao.org/aos/agrovoc/c_11179da9 A linkage map of cacao genome have been constructed using isoenzymes, RFLPOs, and RAPDs markers. A genomic library and cDNA library were constructed from germinated seeds of an Amelonado clone. The screening of probes was made on 5 genotypes, using 5 restriction enzymes. Among 587 tested probes, 52% of cDNA and 44% of genomic probes revealed polymorphic patterns. A population of 100 individuals derived from a cross between two heterozygous genotypes has been used. Segregation analyses were performed with JoinMap programm. The map comprised 193 loci: 5 isoenzymes, 101 cDNA/RFLPs, 4 loci from genes of known fonction, 55 genomic DNA/RFLPs and 28 RAPDs. Ten linkage groups, which putatively correspond to the 10 gametic chromosomes of cacao, have been identified. The map covers a total length of 759 cM with a 3,9 cM average distance between two markers. A small fraction (9%) of the markers deviated significantly from the expected mendelian ratios conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/closedAccess |
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F30 - Génétique et amélioration des plantes Theobroma cacao adn carte génétique RAPD RFLP isoenzyme liaison génétique http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_36341 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_15987 http://aims.fao.org/aos/agrovoc/c_11179da9 F30 - Génétique et amélioration des plantes Theobroma cacao adn carte génétique RAPD RFLP isoenzyme liaison génétique http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_36341 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_15987 http://aims.fao.org/aos/agrovoc/c_11179da9 |
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F30 - Génétique et amélioration des plantes Theobroma cacao adn carte génétique RAPD RFLP isoenzyme liaison génétique http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_36341 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_15987 http://aims.fao.org/aos/agrovoc/c_11179da9 F30 - Génétique et amélioration des plantes Theobroma cacao adn carte génétique RAPD RFLP isoenzyme liaison génétique http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_36341 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_15987 http://aims.fao.org/aos/agrovoc/c_11179da9 Lanaud, Claire Risterucci, Ange-Marie N'Goran, Jeanne A.K. Clément, Didier Flament, Marie-Henriette Laurent, V. A genetic linkage map of Theobroma cacao L. based on isozymes, RFLPs, and RAPDs markers |
description |
A linkage map of cacao genome have been constructed using isoenzymes, RFLPOs, and RAPDs markers. A genomic library and cDNA library were constructed from germinated seeds of an Amelonado clone. The screening of probes was made on 5 genotypes, using 5 restriction enzymes. Among 587 tested probes, 52% of cDNA and 44% of genomic probes revealed polymorphic patterns. A population of 100 individuals derived from a cross between two heterozygous genotypes has been used. Segregation analyses were performed with JoinMap programm. The map comprised 193 loci: 5 isoenzymes, 101 cDNA/RFLPs, 4 loci from genes of known fonction, 55 genomic DNA/RFLPs and 28 RAPDs. Ten linkage groups, which putatively correspond to the 10 gametic chromosomes of cacao, have been identified. The map covers a total length of 759 cM with a 3,9 cM average distance between two markers. A small fraction (9%) of the markers deviated significantly from the expected mendelian ratios |
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conference_item |
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F30 - Génétique et amélioration des plantes Theobroma cacao adn carte génétique RAPD RFLP isoenzyme liaison génétique http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_36341 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_15987 http://aims.fao.org/aos/agrovoc/c_11179da9 |
author |
Lanaud, Claire Risterucci, Ange-Marie N'Goran, Jeanne A.K. Clément, Didier Flament, Marie-Henriette Laurent, V. |
author_facet |
Lanaud, Claire Risterucci, Ange-Marie N'Goran, Jeanne A.K. Clément, Didier Flament, Marie-Henriette Laurent, V. |
author_sort |
Lanaud, Claire |
title |
A genetic linkage map of Theobroma cacao L. based on isozymes, RFLPs, and RAPDs markers |
title_short |
A genetic linkage map of Theobroma cacao L. based on isozymes, RFLPs, and RAPDs markers |
title_full |
A genetic linkage map of Theobroma cacao L. based on isozymes, RFLPs, and RAPDs markers |
title_fullStr |
A genetic linkage map of Theobroma cacao L. based on isozymes, RFLPs, and RAPDs markers |
title_full_unstemmed |
A genetic linkage map of Theobroma cacao L. based on isozymes, RFLPs, and RAPDs markers |
title_sort |
genetic linkage map of theobroma cacao l. based on isozymes, rflps, and rapds markers |
publisher |
s.n. |
url |
http://agritrop.cirad.fr/389712/ |
work_keys_str_mv |
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