A genetic linkage map of Theobroma cacao L. based on isozymes, RFLPs, and RAPDs markers
A linkage map of cacao genome have been constructed using isoenzymes, RFLPOs, and RAPDs markers. A genomic library and cDNA library were constructed from germinated seeds of an Amelonado clone. The screening of probes was made on 5 genotypes, using 5 restriction enzymes. Among 587 tested probes, 52% of cDNA and 44% of genomic probes revealed polymorphic patterns. A population of 100 individuals derived from a cross between two heterozygous genotypes has been used. Segregation analyses were performed with JoinMap programm. The map comprised 193 loci: 5 isoenzymes, 101 cDNA/RFLPs, 4 loci from genes of known fonction, 55 genomic DNA/RFLPs and 28 RAPDs. Ten linkage groups, which putatively correspond to the 10 gametic chromosomes of cacao, have been identified. The map covers a total length of 759 cM with a 3,9 cM average distance between two markers. A small fraction (9%) of the markers deviated significantly from the expected mendelian ratios
| Main Authors: | , , , , , |
|---|---|
| Format: | conference_item biblioteca |
| Language: | eng |
| Published: |
s.n.
|
| Subjects: | F30 - Génétique et amélioration des plantes, Theobroma cacao, adn, carte génétique, RAPD, RFLP, isoenzyme, liaison génétique, http://aims.fao.org/aos/agrovoc/c_7713, http://aims.fao.org/aos/agrovoc/c_2347, http://aims.fao.org/aos/agrovoc/c_24002, http://aims.fao.org/aos/agrovoc/c_36341, http://aims.fao.org/aos/agrovoc/c_34255, http://aims.fao.org/aos/agrovoc/c_15987, http://aims.fao.org/aos/agrovoc/c_11179da9, |
| Online Access: | http://agritrop.cirad.fr/389712/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | A linkage map of cacao genome have been constructed using isoenzymes, RFLPOs, and RAPDs markers. A genomic library and cDNA library were constructed from germinated seeds of an Amelonado clone. The screening of probes was made on 5 genotypes, using 5 restriction enzymes. Among 587 tested probes, 52% of cDNA and 44% of genomic probes revealed polymorphic patterns. A population of 100 individuals derived from a cross between two heterozygous genotypes has been used. Segregation analyses were performed with JoinMap programm. The map comprised 193 loci: 5 isoenzymes, 101 cDNA/RFLPs, 4 loci from genes of known fonction, 55 genomic DNA/RFLPs and 28 RAPDs. Ten linkage groups, which putatively correspond to the 10 gametic chromosomes of cacao, have been identified. The map covers a total length of 759 cM with a 3,9 cM average distance between two markers. A small fraction (9%) of the markers deviated significantly from the expected mendelian ratios |
|---|