Antioxidant capacity, vitamin C and polyphenol profile evaluation of a Capsicum chinense by-product extract obtained by ultrasound using eutectic solvent

Habanero pepper leaves and stems (by-products) have been traditionally considered waste; however, bioactive compounds such as polyphenols, vitamin C and carotenoids have been identified that can be used for formulation of nutraceuticals or functional foods. Furthermore, the extraction of these bioactive compounds by using environmentally friendly methods and solvents is desirable. Thus, the aim of this study was to assess the antioxidant capacity, total polyphenol content (TPC), the phenolic profile and vitamin C content in extracts obtained from by-products (stems and leaves) of two varieties (Mayapan and Jaguar) of habanero pepper by ultrasound-assisted extraction (UAE) using natural deep eutectic solvents (NADES). The results showed that NADES leads to extracts with significantly higher TPC, higher concentrations of individual polyphenols (gallic acid, protocatechuic acid, chlorogenic acid, cinnamic acid, coumaric acid), vitamin C and, finally, higher antioxidant capacity (9.55 ± 0.02 eq mg Trolox/g DM) than UAE extraction performed with methanol as the solvent. The association of individual polyphenols with NADES was confirmed by principal component analysis (PCA). Overall, NADES is an innovative and promising “green” extraction technique that can be applied successfully for the extraction of phenolic compounds from habanero pepper by-products. © 2022 by the authors.

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Bibliographic Details
Main Authors: Kevin Alejandro Avilés Betanzos, Julio Enrique Oney Montalvo, JUAN VALERIO CAUICH RODRIGUEZ, MARISELA GONZALEZ AVILA, Matteo Scampicchio, Ksenia Morozova, MANUEL OCTAVIO RAMIREZ SUCRE, Ingrid Mayanin Rodríguez Buenfil
Format: info:eu-repo/semantics/article biblioteca
Language:eng
Subjects:info:eu-repo/classification/Autores/CAPSICUM CHINENSE, info:eu-repo/classification/Autores/BY-PRODUCT, info:eu-repo/classification/Autores/ANTIOXIDANT CAPACITY, info:eu-repo/classification/Autores/POLYPHENOLS, info:eu-repo/classification/Autores/NATURAL DEEP EUTECTIC SOLVENT, info:eu-repo/classification/Autores/NADES, info:eu-repo/classification/cti/7, info:eu-repo/classification/cti/33, info:eu-repo/classification/cti/3312, info:eu-repo/classification/cti/331208,
Online Access:http://cicy.repositorioinstitucional.mx/jspui/handle/1003/2256
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Summary:Habanero pepper leaves and stems (by-products) have been traditionally considered waste; however, bioactive compounds such as polyphenols, vitamin C and carotenoids have been identified that can be used for formulation of nutraceuticals or functional foods. Furthermore, the extraction of these bioactive compounds by using environmentally friendly methods and solvents is desirable. Thus, the aim of this study was to assess the antioxidant capacity, total polyphenol content (TPC), the phenolic profile and vitamin C content in extracts obtained from by-products (stems and leaves) of two varieties (Mayapan and Jaguar) of habanero pepper by ultrasound-assisted extraction (UAE) using natural deep eutectic solvents (NADES). The results showed that NADES leads to extracts with significantly higher TPC, higher concentrations of individual polyphenols (gallic acid, protocatechuic acid, chlorogenic acid, cinnamic acid, coumaric acid), vitamin C and, finally, higher antioxidant capacity (9.55 ± 0.02 eq mg Trolox/g DM) than UAE extraction performed with methanol as the solvent. The association of individual polyphenols with NADES was confirmed by principal component analysis (PCA). Overall, NADES is an innovative and promising “green” extraction technique that can be applied successfully for the extraction of phenolic compounds from habanero pepper by-products. © 2022 by the authors.