In vitro and in vivo digestibility of prebiotic galactooligosacharides synthesized by β-galactosidase from Lactobacillus delbruecki subsp. bulgaricus CRL450

[Background] The general assumption that prebiotics reach the colon without any alterations has been challenged. Some in vitro and in vivo studies have demonstrated that 'non-digestible' oligosaccharides are digested to different degrees depending on their structural composition. In the present study, we compared different methods aiming to assess the digestibility of oligosaccharides synthesized by β-galactosidase (β-gal) of Lactobacillus delbruecki subsp. bulgaricus CRL450 (CRL450-β-gal) from lactose, lactulose and lactitol.

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Bibliographic Details
Main Authors: Fara, Agustina, Hernández-Hernández, Oswaldo, Palacios, Jorge, Montilla, Antonia, Zárate, Gabriela
Other Authors: CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)
Format: artículo biblioteca
Language:English
Published: Wiley-VCH 2024
Subjects:Lactobacillus delbruecki subsp. bulgaricus, Oligosaccharides, Brush border membrane vesicles (BBMV), In vivo digestion, Rat small intestinal extract (RSIE),
Online Access:http://hdl.handle.net/10261/372879
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Summary:[Background] The general assumption that prebiotics reach the colon without any alterations has been challenged. Some in vitro and in vivo studies have demonstrated that 'non-digestible' oligosaccharides are digested to different degrees depending on their structural composition. In the present study, we compared different methods aiming to assess the digestibility of oligosaccharides synthesized by β-galactosidase (β-gal) of Lactobacillus delbruecki subsp. bulgaricus CRL450 (CRL450-β-gal) from lactose, lactulose and lactitol.