Casein and whey protein in vitro digestion in comparison with duodenal and jejunal in vivo digests

Casein and whey protein in vitro digestion in comparison with duodenal and jejunal in vivo digests

Resumen del trabajo presentado a la 5th International Conference on Food Digestion, celebrada en Rennes (Francia) del 4 al 6 de abril de 2017.

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Main Authors: Sanchón, Javier, Miralles, Beatriz, Pastor, Irene, Sánchez-Rivera, Laura, Amigo, Lourdes, Dupont, Didier, Tomé, Daniel, Gaudichon, Claire, Recio, Isidra
Format: comunicación de congreso biblioteca
Language:English
Published: 2017
Online Access:http://hdl.handle.net/10261/195811
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spelling dig-cial-es-10261-1958112019-12-03T01:52:57Z Casein and whey protein in vitro digestion in comparison with duodenal and jejunal in vivo digests Sanchón, Javier Miralles, Beatriz Pastor, Irene Sánchez-Rivera, Laura Amigo, Lourdes Dupont, Didier Tomé, Daniel Gaudichon, Claire Recio, Isidra Resumen del trabajo presentado a la 5th International Conference on Food Digestion, celebrada en Rennes (Francia) del 4 al 6 de abril de 2017. The COST Action INFOGEST has developed a harmonized static in vitro digestion protocol based on physiologically relevant conditions. This method has already proved to produce comparable results in inter-laboratory trials. Since peptides generated during protein digestion might be biologically relevant for different physiological functions, it is important to directly compare the in vitro results towards in vivo data at peptide level. Therefore, the objective of this work was to assess the casein and whey proteins in vitro digestion according to the harmonized protocol and to match the outcomes with in vivo digests from pig duodenum (with in vitro gastric phase), and human jejunum (with the in vítro intestinal phase). Protein.degradation was followed by SDS-PAGE and the HPLC-MS/MS sequenced peptides were processed using peptidomic and statistic tools. In pig duodenum, small amounts of intact caseins were present in all samples, while intact caseins were observed up to 60 minutes of gastric in vitro digestion. Regarding whey proteins, β-lactoglobulin could be detected in human jejunal digests taken at 60 minutes after ingestion, and similarly, this protein was hydrolysed after 60 min into contact with the simulated intestinal juice. The peptide profiles generated after in vítro and in vivo digestions showed clear similarities with characteristic overrepresented regions in all milk proteins. For instance, N-terminal and C-terminal domains of β-casein, and αs1-casein regions 23-30 and 183-193, were the source of abundant peptides, in both, in vitro and in vivo digests. Based on the peptide profiles, the correlation coefficients calculated between in vítro digests and the human jejunal effluents were within the range found for the different volunteers of the study. Results indicate that the harmonized in vítro protocol is a robust and simple model and constitutes a good approximation to the physiological digestion of milk proteins. Peer reviewed 2019-12-02T10:29:36Z 2019-12-02T10:29:36Z 2017 comunicación de congreso http://purl.org/coar/resource_type/c_5794 5th International Conference on Food Digestion (2017) http://hdl.handle.net/10261/195811 en Sí none
institution CIAL ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-cial-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del CIAL España
language English
description Resumen del trabajo presentado a la 5th International Conference on Food Digestion, celebrada en Rennes (Francia) del 4 al 6 de abril de 2017.
format comunicación de congreso
author Sanchón, Javier
Miralles, Beatriz
Pastor, Irene
Sánchez-Rivera, Laura
Amigo, Lourdes
Dupont, Didier
Tomé, Daniel
Gaudichon, Claire
Recio, Isidra
spellingShingle Sanchón, Javier
Miralles, Beatriz
Pastor, Irene
Sánchez-Rivera, Laura
Amigo, Lourdes
Dupont, Didier
Tomé, Daniel
Gaudichon, Claire
Recio, Isidra
Casein and whey protein in vitro digestion in comparison with duodenal and jejunal in vivo digests
author_facet Sanchón, Javier
Miralles, Beatriz
Pastor, Irene
Sánchez-Rivera, Laura
Amigo, Lourdes
Dupont, Didier
Tomé, Daniel
Gaudichon, Claire
Recio, Isidra
author_sort Sanchón, Javier
title Casein and whey protein in vitro digestion in comparison with duodenal and jejunal in vivo digests
title_short Casein and whey protein in vitro digestion in comparison with duodenal and jejunal in vivo digests
title_full Casein and whey protein in vitro digestion in comparison with duodenal and jejunal in vivo digests
title_fullStr Casein and whey protein in vitro digestion in comparison with duodenal and jejunal in vivo digests
title_full_unstemmed Casein and whey protein in vitro digestion in comparison with duodenal and jejunal in vivo digests
title_sort casein and whey protein in vitro digestion in comparison with duodenal and jejunal in vivo digests
publishDate 2017
url http://hdl.handle.net/10261/195811
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