Green-based methods to obtain bioactive extracts from Plantago major and Plantago lanceolata
P. major and P. lanceolata are two medicinal plants used extensively all over the world as remedies for a wide range of diseases. This study aimed to apply a sustainable recovery extraction to obtain bioactive extracts from the medicinal plants P. major and P. lanceolata. Different extraction methods were compared in terms of yield process, total phenolic content (TPC) and antioxidant and antibacterial activities. The methods used were: supercritical fluid extraction (SFE) with CO pure and with ethanol as co-solvent, conducted at temperatures from 40 to 60 °C and pressures from 10 to 30 MPa, and by Soxhlet (SOX) and ultrasound-assisted extraction (UE) with different solvents. High extraction yields were obtained by SOX with methanol (P. major) and by UE with EtOH-HO (P. lanceolata). The moderate-polar and polar solvents presented the best TPC values and antioxidant performance, included the supercritical extraction of P. major with 5.0% of ethanol as co-solvent (EC 276 ± 1). SC-CO extract presented the best antibacterial activity, being more effective against the Gram-positive bacteria (Bacillus cereus). The results showed that the use of green recovery methods such as SFE and UE with polar solvents (EtOH-HO and ethanol) provide high valuable extracts for Plantago species.
| Main Authors: | , , |
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| Other Authors: | |
| Format: | artículo biblioteca |
| Published: |
Elsevier
2017
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| Subjects: | Supercritical extraction, Plantago species, Total phenolic content, Ultrasound-assisted extraction, |
| Online Access: | http://hdl.handle.net/10261/150573 http://dx.doi.org/10.13039/501100007082 http://dx.doi.org/10.13039/501100002322 |
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| Summary: | P. major and P. lanceolata are two medicinal plants used extensively all over the world as remedies for a wide range of diseases. This study aimed to apply a sustainable recovery extraction to obtain bioactive extracts from the medicinal plants P. major and P. lanceolata. Different extraction methods were compared in terms of yield process, total phenolic content (TPC) and antioxidant and antibacterial activities. The methods used were: supercritical fluid extraction (SFE) with CO pure and with ethanol as co-solvent, conducted at temperatures from 40 to 60 °C and pressures from 10 to 30 MPa, and by Soxhlet (SOX) and ultrasound-assisted extraction (UE) with different solvents. High extraction yields were obtained by SOX with methanol (P. major) and by UE with EtOH-HO (P. lanceolata). The moderate-polar and polar solvents presented the best TPC values and antioxidant performance, included the supercritical extraction of P. major with 5.0% of ethanol as co-solvent (EC 276 ± 1). SC-CO extract presented the best antibacterial activity, being more effective against the Gram-positive bacteria (Bacillus cereus). The results showed that the use of green recovery methods such as SFE and UE with polar solvents (EtOH-HO and ethanol) provide high valuable extracts for Plantago species. |
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