A Foodomics approach: CE-MS for comparative metabolomics of colon cancer cells treated with dietary polyphenols
The potential of capillary electrophoresis-mass spectrometry (CE-MS) for metabolomics is demonstrated through the analysis of metabolites from human HT29 colon cancer cells treated and non-treated with dietary polyphenols. Prior to CE-MS analysis, four different metabolite purification strategies are investigated. Namely, the results obtained after methanol deproteinization, ultrafiltration, and two solid-phase extraction methods using C18 and polymer-based cartridges are described. These generic methods can have broad applications to analyze metabolites in a large variety of matrices and fields, including the new Foodomics area.
Saved in:
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | capítulo de libro biblioteca |
| Published: |
Springer
2012
|
| Subjects: | Colon cancer, Metabolomics, Dietary polyphenols, CE, CE-MS, Foodomics, |
| Online Access: | http://hdl.handle.net/10261/101222 http://dx.doi.org/10.13039/501100004837 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The potential of capillary electrophoresis-mass spectrometry (CE-MS) for metabolomics is demonstrated through the analysis of metabolites from human HT29 colon cancer cells treated and non-treated with dietary polyphenols. Prior to CE-MS analysis, four different metabolite purification strategies are investigated. Namely, the results obtained after methanol deproteinization, ultrafiltration, and two solid-phase extraction methods using C18 and polymer-based cartridges are described. These generic methods can have broad applications to analyze metabolites in a large variety of matrices and fields, including the new Foodomics area. |
|---|