Supercritical and enzymatic technologies for the production of lysophosphatidylcholine
[Background]: This study focuses on obtaining a highly purified lysophosphatidylcholine from phosphatidylcholine powder via hydrolysis, catalyzed by a phospholipase A2 in a solvent-free medium, and subsequent purification either by organic solvent or by supercritical fluid extraction. [Results]: A solvent-free hydrolysis reaction of phosphatidylcholine powder to produce lysophosphatidylcholine with residual phosphatidylcholine content lower than 10% has been described and scaled-up. Supercritical fluid mixtures of carbon dioxide, ethanol and formic acid were studied at 250, bar 50 °C to selectively extract the free fatty acids fraction from hydrolyzed phosphatidylcholine. Finally, a solid product with lysophosphatidylcholine content of c. 95% (w/w) and with free fatty acids content of c. 5% (w/w) was attained. Under optimum conditions, the content of the original phosphatidylcholine in the lysophosphatidylcholine product was lower than 5% (w/w). [Conclusions]: An efficient hydrolysis of phosphatidylcholine by phospholipase A2 in a solvent-free reaction was developed. In addition, three novel strategies for the purification of lysophosphatidylcholine have been developed: (1) hexane/formic acid extraction process, (2) supercritical CO2 extraction with ethanol as modifier, and (3) supercritical CO2 extraction with ethanol/formic acid as modifier. © 2012 Society of Chemical Industry.
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | artículo biblioteca |
| Published: |
Blackwell Publishing
2013
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| Subjects: | Phospholipid, Purification, Supercritical fluid extraction, Phospholipase A2, Hydrolysis, Lysophosphatidylcholine, |
| Online Access: | http://hdl.handle.net/10261/100321 http://dx.doi.org/10.13039/100012818 |
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| Summary: | [Background]: This study focuses on obtaining a highly purified lysophosphatidylcholine from phosphatidylcholine powder via hydrolysis, catalyzed by a phospholipase A2 in a solvent-free medium, and subsequent purification either by organic solvent or by supercritical fluid extraction. [Results]: A solvent-free hydrolysis reaction of phosphatidylcholine powder to produce lysophosphatidylcholine with residual phosphatidylcholine content lower than 10% has been described and scaled-up. Supercritical fluid mixtures of carbon dioxide, ethanol and formic acid were studied at 250, bar 50 °C to selectively extract the free fatty acids fraction from hydrolyzed phosphatidylcholine. Finally, a solid product with lysophosphatidylcholine content of c. 95% (w/w) and with free fatty acids content of c. 5% (w/w) was attained. Under optimum conditions, the content of the original phosphatidylcholine in the lysophosphatidylcholine product was lower than 5% (w/w). [Conclusions]: An efficient hydrolysis of phosphatidylcholine by phospholipase A2 in a solvent-free reaction was developed. In addition, three novel strategies for the purification of lysophosphatidylcholine have been developed: (1) hexane/formic acid extraction process, (2) supercritical CO2 extraction with ethanol as modifier, and (3) supercritical CO2 extraction with ethanol/formic acid as modifier. © 2012 Society of Chemical Industry. |
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