High quality draft genome of Brucella abortus strain Col-B012, isolated from a Holstein cattle in Nariño Colombia, brings new insights into the diagnosis and the epidemiology of biovar 4 strains
Brucellosis is a commonly diagnosed zoonosis that causes infertility and abortion in cattle, it is acquired from handling of infected animals or consuming contaminated milk or milk products. In Colombia, it is classified as prevention and control disease, despite its relevance little is known about the origin, epidemiology and the genetic constituents of the strains circulating in dairy farms. Here we present the draft genome of B. abortus Col-B012, an isolate obtained from a female Holstein belonging to a dairy farm in Nariño, Colombia. This genome comprises 3,234,714 bp and 3,018 predicted protein-encoding genes. Using comparative genomics and phylogenetic analysis, we found that the strain Col-B012 clustered with known biovar 4 variants. The analysis of the core genesallowed the identification of polymorphisms only present in biovar 4 genomes, these alleles might be associated with the phenotypic and pathogenic characteristics of the group and are proposed as possible targets for identification by PCR. The sequencing of B. abortus Col-B012 genome provides important insights to improve the diagnosis and the epidemiology of this disease and represents the first report of the biovar 4 in Colombia.
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | article biblioteca |
| Language: | eng |
| Published: |
Peerj
2017-11-17
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| Subjects: | Brucellosis, Biovar, Genome, Diagnosis, Coparative genomics, Enfermedades de los animales - L73, Ganado bovino, Enfermedades de los animales, Brucelosis, Epidemiología, Ganadería y especies menores, http://aims.fao.org/aos/agrovoc/c_1391, http://aims.fao.org/aos/agrovoc/c_426, http://aims.fao.org/aos/agrovoc/c_1122, http://aims.fao.org/aos/agrovoc/c_2615, |
| Online Access: | https://peerj.com/preprints/3416/ http://hdl.handle.net/20.500.12324/39731 |
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| Summary: | Brucellosis is a commonly diagnosed zoonosis that causes infertility and abortion
in cattle, it is acquired from handling of infected animals or consuming
contaminated milk or milk products. In Colombia, it is classified as prevention and
control disease, despite its relevance little is known about the origin,
epidemiology and the genetic constituents of the strains circulating in dairy farms.
Here we present the draft genome of B. abortus Col-B012, an isolate obtained
from a female Holstein belonging to a dairy farm in Nariño, Colombia. This
genome comprises 3,234,714 bp and 3,018 predicted protein-encoding genes.
Using comparative genomics and phylogenetic analysis, we found that the strain
Col-B012 clustered with known biovar 4 variants. The analysis of the core genesallowed the identification of polymorphisms only present in biovar 4 genomes,
these alleles might be associated with the phenotypic and pathogenic
characteristics of the group and are proposed as possible targets for
identification by PCR. The sequencing of B. abortus Col-B012 genome provides
important insights to improve the diagnosis and the epidemiology of this disease
and represents the first report of the biovar 4 in Colombia. |
|---|