An investigation on artificial reproduction of Mugil cephalus
Cultured nine years old breeder M. cephalus specimens were subjected to eight artificial breeding treatments from December till February 2003. In treatments 1-5, breeders received two injections of CPH and LIIRFI—A2 coupled with Domperidone or a mixture of CPH and HCG in an interval of 24 hours. Female breeders in treatments 6-8 received a gradual daily injection of 500 1U HCG per kilogram of body weight for 5 days. Male breeders in treatments 6-8 were given 5-10mg of MT-a-17 in addition to HCG and then subjected to two injections similar to that of treatments 1-5. Results showed that male breeders in stages +2 and +3 of all treatments that had received HCG produced more milt than those injected with MT-a-17, such that each male was used 2-6 times for milting purposes. Of 27 female breeders, 22 spawned 1-2.6 million eggs among which eight females' eggs were fertilized 10-95%. Hatching rate was between 0.008 to 88.9% and a maximum of 2 million larvae were produced. The best time for artificial breeding is December when mean egg width is ,600 microns. Statistical analysis of egg width, hatching rate and larvae production showed a significant difference between treatments 1-5 and 6-8 (p<0.05). This proved the supremacy of multiple injections including HCG for artificial breeding of Mugil cephalus.
| Main Authors: | , , , , |
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| Format: | article biblioteca |
| Language: | Persian |
| Published: |
2006
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| Subjects: | Aquaculture, Mugil cephalus, Artificial breeding, Iran, |
| Online Access: | http://hdl.handle.net/1834/39106 |
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| Summary: | Cultured nine years old breeder M. cephalus specimens were subjected to eight artificial breeding treatments from December till February 2003. In treatments 1-5, breeders received two injections of CPH and LIIRFI—A2 coupled with Domperidone or a mixture of CPH and HCG in an interval of 24 hours. Female breeders in treatments 6-8 received a gradual daily injection of 500 1U HCG per kilogram of body weight for 5 days. Male breeders in treatments 6-8 were given 5-10mg of MT-a-17 in addition to HCG and then subjected to two injections similar to that of treatments 1-5. Results showed that male breeders in stages +2 and +3 of all treatments that had received HCG produced more milt than those injected with MT-a-17, such that each male was used 2-6 times for milting purposes. Of 27 female breeders, 22 spawned 1-2.6 million eggs among which eight females' eggs were fertilized 10-95%. Hatching rate was between 0.008 to 88.9% and a maximum of 2 million larvae were produced. The best time for artificial breeding is December when mean egg width is ,600 microns. Statistical analysis of egg width, hatching rate and larvae production showed a significant difference between treatments 1-5 and 6-8 (p<0.05). This proved the supremacy of multiple injections including HCG for artificial breeding of Mugil cephalus. |
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