Molecular identification of Vibrio harveyi from larval stage of Pacific white shrimp (Litopenaeus vannamei) Boone (Crustacea:Decapoda) by polymerase chain reaction and 16S rDNA sequencing
A Gram-negative, rod-shaped, motile and halophilic bacterium identified as Vibrio harveyi was isolated from hatchery-reared larval of Iran Shrimp Research Center following mass mortality. Seven isolates of Vibrio spp. were collected from nauplii of Litopenaeus vannamei and then were evaluated for characteristics including morphology, physiology and biochemistry. The results indicated that the isolates belonged to a single species that grew on Thiosulfate citrate bile sucrose agar and in 1.5 to 6% NaCl, positive lysine and ornithine decarboxylation and susceptible to O/129 disk. Identification of Vibrio harveyi strainIS01 (1 of 7 isolates) was confirmed by Polymerase Chain Reaction assay for Vibrio harveyi (expected amplicon 1493 bp). The 16S ribosomal DNA sequence (National Center for Biotechnology Information, GenBank accession number GU974342.1) gave 99% sequence identity to V. harveyi ATCC: 35084 (GenBank accession number EU130475.1).
| Main Authors: | , , , , |
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| Format: | article biblioteca |
| Language: | English |
| Published: |
2014
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| Subjects: | Aquaculture, Biology, Fisheries, Vibriosis, shrimp diseases, Litopenaeus vannamei, 16S rDNA sequencing, Iran, Pacific white shrimp, Larval, Vibrio harveyi, Molecular identification, |
| Online Access: | http://hdl.handle.net/1834/37449 |
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| Summary: | A Gram-negative, rod-shaped, motile and halophilic bacterium identified as Vibrio harveyi was isolated from hatchery-reared larval of Iran Shrimp Research Center following mass mortality. Seven isolates of Vibrio spp. were collected from nauplii of Litopenaeus vannamei and then were evaluated for characteristics including morphology, physiology and biochemistry. The results indicated that the isolates belonged to a single species that grew on Thiosulfate citrate bile sucrose agar and in 1.5 to 6% NaCl, positive lysine and ornithine decarboxylation and susceptible to O/129 disk. Identification of Vibrio harveyi strainIS01 (1 of 7 isolates) was confirmed by Polymerase Chain Reaction assay for Vibrio harveyi (expected amplicon 1493 bp). The 16S ribosomal DNA sequence (National Center for Biotechnology Information, GenBank accession number GU974342.1) gave 99% sequence identity to V. harveyi ATCC: 35084 (GenBank accession number EU130475.1). |
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