Caracterización molecular de rayas simpátricas de los géneros Dipturus y Zearaja en el Mar Argentino por DNA barcode.
Skates of the genera Dipturus and Zearaja present a particular challenge for fishery management and conservation, since they have been subject to intense exploitation in the last years. In the Argentine Sea, these genera are represented by D. leptocauda, D. trachyderma, D. argentinensis and Z. chilensis. These last three are sympatric species since they overlap in depth and geographic distribution. Their behavioral habits and the morphological similarity make these species, especially in juvenile stages, difficult to identify. The use of the mitochondrial gene Cytochrome C Oxidase I (COI) as a DNA Barcode (genetic barcode), is a useful molecular tool to identify morphologically similar species. In this work, we used the DNA Barcode to identify specimens of Z. chilensis that presented a different thorny pattern in their dorsal side and tail instead of the common pattern found in that species. Also, we compared the DNA Barcode with other sympatric skates' species to obtain a molecular characterization of the Dipturus and Zearaja. The DNA Barcode was obtained from 23 individuals collected along the Argentine continental shelf. The specimens were classified as D. argentinesis, Z. chilensis and Z. chilensis with a distinct different spinal pattern (specimens ZSP). The specimens were identified at species level by comparing their barcodes with reference sequences of specimens from the Pacific and Atlantic ocean in Barcode of Life Data System (BOLD), and also by the intra and interspecific Kimura two-parameter (K2P) distance. From this work, we conclude that the DNA Barcode is a useful and efficient molecular marker to identify skates' species, and is a useful tool as a reference parameter for the differentiation of the species.
| Main Authors: | , , , |
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| Format: | Journal Contribution biblioteca |
| Language: | Spanish / Castilian |
| Published: |
2017
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| Subjects: | ADN, Código de barras ADN, Secuencia de ADN, Identificación de especies, Rajidae, Dipturus argentinensis, Zearaja chilensis, Raya, DNA, DNA barcoding, DNA sequencing, Species identification, |
| Online Access: | http://hdl.handle.net/1834/14786 |
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| Summary: | Skates of the genera Dipturus and Zearaja present a particular challenge for fishery management and conservation, since they have been subject to intense exploitation in the last years. In the Argentine Sea, these genera are represented by D. leptocauda, D. trachyderma, D. argentinensis and Z. chilensis. These last three are sympatric species since they overlap in depth and geographic distribution. Their behavioral habits and the morphological similarity make these species, especially in juvenile stages, difficult to identify. The use of the mitochondrial gene Cytochrome C Oxidase I (COI) as a DNA Barcode (genetic barcode), is a useful molecular tool to identify morphologically similar species. In this work, we used the DNA Barcode to identify specimens of Z. chilensis that presented a different thorny pattern in their dorsal side and tail instead of the common pattern found in that species. Also, we compared the DNA Barcode with other sympatric skates' species to obtain a molecular characterization of the Dipturus and Zearaja. The DNA Barcode was obtained from 23 individuals collected along the Argentine continental shelf. The specimens were classified as D. argentinesis, Z. chilensis and Z. chilensis with a distinct different spinal pattern (specimens ZSP). The specimens were identified at species level by comparing their barcodes with reference sequences of specimens from the Pacific and Atlantic ocean in Barcode of Life Data System (BOLD), and also by the intra and interspecific Kimura two-parameter (K2P) distance. From this work, we conclude that the DNA Barcode is a useful and efficient molecular marker to identify skates' species, and is a useful tool as a reference parameter for the differentiation of the species. |
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