Optimization of growth, lipid and chlorophyll contents in Chlorella vulgaris under different conditions of nitrate, phosphate and photoperiod by central composite design (CCD)
The effects of nitrate (750-2250 mg/L), phosphate (20-60 mg/L) and photoperiod (8-24 h) on the growth, chlorophyll content and lipid yield of Chlorella vulgaris were studied using central composite design (CCD) method. The second-order quadratic model was used to predict the effects of input variables on the output responses and the results were verified by F test, ANOVA and regression analyses. Results showed that maximum growth (255.88 ×106 ) as well as highest chlorophyll content (15.34%) were observed at 16 h of light, 1500 mg/L of nitrate and 40 mg/L of phosphate concentration. Also, condition of 24 h of light, 750 mg/L nitrate and 20 mg/L phosphate concentrations resulted in maximum percent of lipid content (17.74%). The optimization of culture condition for simultaneous achievement of highest levels of growth, chlorophyll and lipid content were investigated.
| Main Authors: | , |
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| Format: | Journal Contribution biblioteca |
| Language: | Persian |
| Published: |
2018
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| Subjects: | Environmental and nutritional conditions, Chlorella vulgaris, Biochemical components, Optimization, Growth, Lipid, Chlorophyll, Phosphate, Photoperiod, CCD, |
| Online Access: | http://hdl.handle.net/1834/14636 |
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| Summary: | The effects of nitrate (750-2250 mg/L), phosphate (20-60 mg/L) and photoperiod (8-24 h) on the
growth, chlorophyll content and lipid yield of Chlorella vulgaris were studied using central composite
design (CCD) method. The second-order quadratic model was used to predict the effects of input
variables on the output responses and the results were verified by F test, ANOVA and regression
analyses. Results showed that maximum growth (255.88 ×106
) as well as highest chlorophyll content
(15.34%) were observed at 16 h of light, 1500 mg/L of nitrate and 40 mg/L of phosphate
concentration. Also, condition of 24 h of light, 750 mg/L nitrate and 20 mg/L phosphate
concentrations resulted in maximum percent of lipid content (17.74%). The optimization of culture
condition for simultaneous achievement of highest levels of growth, chlorophyll and lipid content
were investigated. |
|---|