Investigation of Indian white shrimp ( penaeus indicus ) Populations in west and East of Jask area by genetical method
The objective of this investigation was molecular study of Penaeus indicus population from Jask area. Samples were collected using bottem trawl net from three regions, East, West and Central Jask. DNA were extracted using phenol - chloroform method from muscular tissue. To investigate population differences of P. indicus, the specific primers were designed by sequence of Cytochrom oxidase I (CO I) gen in mtDNA molecule. PCR was performed using two spesific primers and the reasonable result achieved (600bp) for all samples. The PCR products of samples were digested by restriction endonuclease enzymes such as (Alu I, and followed by polyacrilamid gel Taq I, Hinf I, Rsa I, Dde I, Hinc II, Hind III, Hpa II, Ecor I, Pvu II) Observed by silver nitrate staining method. electrophoresis (PAGE) The bands of DNA were the same in all samples and the results indicated no polymorphism between samples. These results may suggested that cytochrom oxidase I gen in Penaeus indicus is a conserved gen or numbers of restriction endonuclease enzymes enough for the study of genetic variations in or all samples are belong to a single genetic population and was no possible for isolation of different genetic populations in this specie. Maybe the East, West and Central Jask are not sofar from eachother and absence of any Human made or different Biology in these sites are expected and no changes in genetic level.
| Main Authors: | , |
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| Format: | Report biblioteca |
| Language: | Persian |
| Published: |
Iranian Fisheries Science Research Institute
2005
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| Subjects: | mtDNA, PCR, RFLP, Penaeus indicus, Gentic variation, Cytochrom Oxidase I, Molecular, Population, |
| Online Access: | http://hdl.handle.net/1834/14385 |
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| Summary: | The objective of this investigation was molecular study of Penaeus indicus population from Jask
area. Samples were collected using bottem trawl net from three regions, East, West and Central Jask. DNA were extracted using phenol - chloroform method from muscular tissue. To investigate
population differences of P. indicus, the specific primers were designed by sequence of Cytochrom
oxidase I (CO I) gen in mtDNA molecule. PCR was performed using two spesific primers and the reasonable result achieved (600bp) for all
samples. The PCR products of samples were digested by restriction endonuclease enzymes such as (Alu I, and followed by polyacrilamid gel Taq I, Hinf I, Rsa I, Dde I, Hinc II, Hind III, Hpa II, Ecor I, Pvu II)
Observed by silver nitrate staining method. electrophoresis (PAGE)
The bands of DNA were the same in all samples and the results indicated no polymorphism
between samples. These results may suggested that cytochrom oxidase I gen in Penaeus indicus is a
conserved gen or numbers of restriction endonuclease enzymes enough for the study of genetic
variations in or all samples are belong to a single genetic population and was no possible for isolation
of different genetic populations in this specie. Maybe the East, West and Central Jask are not sofar
from eachother and absence of any Human made or different Biology in these sites are expected and
no changes in genetic level. |
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