Nutritional requirements to increase the survival rate of black-lip pearl oyster, Pinctada margaritifera from D-shape to spate stage
Black lip pearl oyster (pinctada magaritifera) is one of the most important species of Pearl maker in the world. Its reserves in the Persian Gulf waters is facing to a variety of causes, especially oil pollution from Persian Gulf war that cause genetic changes in pearl oyster. 65 broodstocks black lip pearl oyster mature with average length of the dorsal ventral (15 ± 2cm DVM) were collected from its typical natural Lavan Island in the months of June and July 2007 and were transferred to the research station and then were washed in cold rooms (20 ° C). The gonad indices were determined by microscope based on the gametogenez steps. After 2 days from collection time, the broodstocks were exposed to thermal shock in order of spawning stimulation. For the analysis of biochemical compounds, microalgae were sampled at logarithmic phase stage with three replications. Firstly, the samples were concentrated by centrifuges (3500g, 20 min). After washing with ammonium formate solution 0.5 molar, the samples were again centrifuged at 3500 g for 15 min. After spawning, D shape larvae form and then the larva ambo feeded from different micro-algae and lipid and the amount of growth and survival rates were studied. D shape larvae with 3 larvae per ml density and dorsal- abdominal length of 78.9 ± 0.7 μm were cultured in 5 liter Erlenmeyer flask containing 2 liters water. Whereas, samples at umbo stage with average dorsal-ventral length of 133 ± 3.1 μm were cultured in 15 liters plastic containers at 27- 29 ° C and salinity of 34 to 35 parts per thousand. The studied microalgae were Isochrysis aff galbana, Chaetoceros calcitrans and c.muelleri. The results of biochemical compounds showed that protein is the most component of all species with a maximum amount of 527.5 ± 2.1 mg g-1 in T. Iso microalgae. Also, T. Iso has a maximum amount of lipids, following by seek c. muelleri and c. calcitrans, respectively. Test results showed that feeding of larvae black lip pearl oyster in stage D shape with microalgae T. Iso alone has more growth rate than double or triple mixtures. However, diatoms have a high nutritional value for larval mussels in umbo state, and they are accounted as important components in a double and triple mixture of microalgae diet. The size of the larval D shape after 10 days showed a difference among treatments. The cultured larves that feeded from T Iso reached to maximum length of 111.4 ± 10 μm and the maximum survival rate of 57.7% was related to larves that feeded by fresh microalgae T. Iso. D.V.M for microalgae with microalgae or lipid nutrition showed no significant difference.
| Main Authors: | , , , , , , , , , , , |
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| Format: | Report biblioteca |
| Language: | Persian |
| Published: |
Iranian Fisheries Science Research Institute
2016
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| Subjects: | Pinctada margartifera, Black lip Pearl Oyster, Microalgae, Fatty acids, D shape larvae, Umbo larvae, Dorsal, Ventral measurement, Chaetoceros calcitrans, Survival rate, |
| Online Access: | http://hdl.handle.net/1834/13899 |
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| Summary: | Black lip pearl oyster (pinctada magaritifera) is one of the most important species of Pearl maker in the world.
Its reserves in the Persian Gulf waters is facing to a variety of causes, especially oil pollution from Persian Gulf
war that cause genetic changes in pearl oyster. 65 broodstocks black lip pearl oyster mature with average length
of the dorsal ventral (15 ± 2cm DVM) were collected from its typical natural Lavan Island in the months of June
and July 2007 and were transferred to the research station and then were washed in cold rooms (20 ° C). The
gonad indices were determined by microscope based on the gametogenez steps. After 2 days from collection
time, the broodstocks were exposed to thermal shock in order of spawning stimulation. For the analysis of
biochemical compounds, microalgae were sampled at logarithmic phase stage with three replications. Firstly, the
samples were concentrated by centrifuges (3500g, 20 min). After washing with ammonium formate solution 0.5
molar, the samples were again centrifuged at 3500 g for 15 min. After spawning, D shape larvae form and then
the larva ambo feeded from different micro-algae and lipid and the amount of growth and survival rates were
studied. D shape larvae with 3 larvae per ml density and dorsal- abdominal length of 78.9 ± 0.7 μm were
cultured in 5 liter Erlenmeyer flask containing 2 liters water. Whereas, samples at umbo stage with average
dorsal-ventral length of 133 ± 3.1 μm were cultured in 15 liters plastic containers at 27- 29 ° C and salinity of 34
to 35 parts per thousand. The studied microalgae were Isochrysis aff galbana, Chaetoceros calcitrans and
c.muelleri. The results of biochemical compounds showed that protein is the most component of all species with
a maximum amount of 527.5 ± 2.1 mg g-1 in T. Iso microalgae. Also, T. Iso has a maximum amount of lipids,
following by seek c. muelleri and c. calcitrans, respectively. Test results showed that feeding of larvae black lip
pearl oyster in stage D shape with microalgae T. Iso alone has more growth rate than double or triple mixtures.
However, diatoms have a high nutritional value for larval mussels in umbo state, and they are accounted as
important components in a double and triple mixture of microalgae diet. The size of the larval D shape after 10
days showed a difference among treatments. The cultured larves that feeded from T Iso reached to maximum
length of 111.4 ± 10 μm and the maximum survival rate of 57.7% was related to larves that feeded by fresh
microalgae T. Iso. D.V.M for microalgae with microalgae or lipid nutrition showed no significant difference. |
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