Development gene data bank of cultured shrimp species and their indigenous pathogens in Iran
Protection authorities of plant and animal genetic resources and conservation of biodiversity as national assets by using biotechnology techniques is the most important objectives of genetic data information bank. Establishment of shrimp species genetic data bank, makes possibility of study multiple genes such as economic genes and since knowledge of genetic variation within and among populations and barcoding species of shrimp are very important for species conservation and with so little researches of Asia in the global studies of DNA barcoding and animal taxonomy in Iran, In this study, Iran shrimp genetic diversity and genetic data bank was done. DNA barcode is a short, standard well known sequence of cytochrome oxidase І gene. By using this DNA sequence can be realized that each animal, plant or fungus belongs to which species. Also prepare a bank of genetic data shrimp pathogens and maintenance of these strains are steps to advance future researches in the fields of pathogenesis mechanism of pathogens, diagnosis, treatment, Disease prevention, production diagnosis native kits and detection of emerging and reemerging diseases its origins. So in this plan samples were collected from imported cultured shrimp Litopenaeus vannamei and and 6 Persian Gulf and Oman Sea shrimp species which classified based on traditional systematically as: Penaeus semisulcatus, Fenneropenaeus merguiensis, Metapenaeus affinis, Parapenaeopsis Stylifera and Fenneropenaeus indicus. After examination of DNA barcode sequence, molecular and bioinformatics operations of each sequence in the Consortium for the Barcode of Life (CBOL) and National Center for Biotechnology Information (NCBI), phylogenetic analysis of each sample was determined and similarity of each sample with NCBI and CBOL database was checked and the closest species to each sample were specified. According to the results different samples of L. vannamei, . banded P.semisulcatus, F. merguiensis and F. indicus have more than 97% similarity to the same species of other countries. non banded P.semisulcatus had 80.07% similarity to banded P.semisulcatus, M. affinis samples had 90.3% similarity to Metapenaeus ensis and Parap. Stylifera had 93.44% similarity to Parapenaeopsis coromandelica in the CBOL. This funding confirmed the need for further investigation and possible announcement of new species. By using ribotyping technique, native isolated pathogenic bacteria and fungi were identified and recorded in the gene bank database center. During sampling of shrimp and water of Specific Pathogen Free shrimp center, 40 bacterial strains were isolated, which 8 of them had the most frequency and identification based on 16S rDNA sequencing was performed. Bacteria identified are: Vibrio nigripulchritudo strain IS013(GenBank:KP843725) ،Vibrio brasiliensis strain IS014 (GenBank:KR186076) ،Vibrio rotiferianus strain IS015 (GenBank:KR186077) ،Vibrio azureus strain IS012 (GenBank:KJ018724.1) ،Vibrio owensii strain IS016 (GenBank:KR186078) ،Agarivorans gilvus strain IS017 (GenBank:KR186079) ،Vibrio brasiliensis IS018 (GenBank:KR186080) and Vibrio alginolyticus strain IS019 (GenBank:1817854), which were recorded in The World Bank genes. In this study fungal isolates were not detected.
| Main Authors: | , , , |
|---|---|
| Format: | Report biblioteca |
| Language: | Persian |
| Published: |
Iranian Fisheries Science Research Institute
2016
|
| Subjects: | Bacteria, Ribotyping, 16S rDNA, Shrimp, DNA barcode, GenBank, Vibrio nigripulchritudo, Vibrio rotiferianus, Vibrio owensii, Vibrio brasiliensis, Vibrio azureus, Agarivorans gilvus, Vibrio alginolyticus, Parapenaeopsis coromandelica, Metapenaeus ensis, Litopenaeus vannamei, |
| Online Access: | http://hdl.handle.net/1834/13893 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Protection authorities of plant and animal genetic resources and conservation of biodiversity as national assets by
using biotechnology techniques is the most important objectives of genetic data information bank. Establishment
of shrimp species genetic data bank, makes possibility of study multiple genes such as economic genes and since
knowledge of genetic variation within and among populations and barcoding species of shrimp are very
important for species conservation and with so little researches of Asia in the global studies of DNA barcoding
and animal taxonomy in Iran, In this study, Iran shrimp genetic diversity and genetic data bank was done. DNA
barcode is a short, standard well known sequence of cytochrome oxidase І gene. By using this DNA sequence
can be realized that each animal, plant or fungus belongs to which species. Also prepare a bank of genetic data
shrimp pathogens and maintenance of these strains are steps to advance future researches in the fields of
pathogenesis mechanism of pathogens, diagnosis, treatment, Disease prevention, production diagnosis native kits
and detection of emerging and reemerging diseases its origins. So in this plan samples were collected from
imported cultured shrimp Litopenaeus vannamei and and 6 Persian Gulf and Oman Sea shrimp species which
classified based on traditional systematically as: Penaeus semisulcatus, Fenneropenaeus merguiensis,
Metapenaeus affinis, Parapenaeopsis Stylifera and Fenneropenaeus indicus. After examination of DNA barcode
sequence, molecular and bioinformatics operations of each sequence in the Consortium for the Barcode of Life
(CBOL) and National Center for Biotechnology Information (NCBI), phylogenetic analysis of each sample was
determined and similarity of each sample with NCBI and CBOL database was checked and the closest species to
each sample were specified. According to the results different samples of L. vannamei, . banded P.semisulcatus,
F. merguiensis and F. indicus have more than 97% similarity to the same species of other countries. non banded
P.semisulcatus had 80.07% similarity to banded P.semisulcatus, M. affinis samples had 90.3% similarity to
Metapenaeus ensis and Parap. Stylifera had 93.44% similarity to Parapenaeopsis coromandelica in the CBOL.
This funding confirmed the need for further investigation and possible announcement of new species. By using
ribotyping technique, native isolated pathogenic bacteria and fungi were identified and recorded in the gene bank
database center. During sampling of shrimp and water of Specific Pathogen Free shrimp center, 40 bacterial
strains were isolated, which 8 of them had the most frequency and identification based on 16S rDNA sequencing
was performed. Bacteria identified are:
Vibrio nigripulchritudo strain IS013(GenBank:KP843725) ،Vibrio brasiliensis strain IS014
(GenBank:KR186076) ،Vibrio rotiferianus strain IS015 (GenBank:KR186077) ،Vibrio azureus strain IS012
(GenBank:KJ018724.1) ،Vibrio owensii strain IS016 (GenBank:KR186078) ،Agarivorans gilvus strain IS017
(GenBank:KR186079) ،Vibrio brasiliensis IS018 (GenBank:KR186080) and Vibrio alginolyticus strain IS019
(GenBank:1817854), which were recorded in The World Bank genes. In this study fungal isolates were not
detected. |
|---|