Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants.

Lasiodiplodia theobromae is a major pathogen of many different crop cultures, including cashew nut plants. This paper describes an efficient Agrobacterium tumefaciens-mediated transformation (ATMT) system for the successful delivery of T-DNA, transferring the genes of green fluorescent protein (gfp) and hygromycin B phosphotransferase (hph) to L. theobromae. When the fungal pycnidiospores were co-cultured with A. tumefaciens harboring the binary vector with hph-gfp gene, hygromycin-resistant fungus only developed with acetosyringone supplementation.

Bibliographic Details

Main Authors:	MUNIZ, C. R., SILVA, G. F. da, SOUZA JUNIOR, M. T., FREIRE, F. das C. O., KEMA, G. H. J., GUEDES, M. I. F.
Other Authors:	CELLI RODRIGUES MUNIZ, CNPAT; GILVAN FERREIRA DA SILVA, CPAA; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE; FRANCISCO DAS CHAGAS O FREIRE, CNPAT; G. H. J. Kema, Biointeractions and Plant Health; M. I. F. Guedes, Universidade Estadual do Ceará.
Format:	Separatas biblioteca
Language:	English eng
Published:	2014-05-12
Subjects:	Cashew plants, Mediated transformation., Agrobacterium Tumefaciens, Lasiodiplodia Theobromae., gummosis.,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/985915
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