Simultaneous detection and identification of the Xanthomonas species complex associated with tomato bacterial spot using species-specifics primers and multiples PCR.
To establish protocols for the simultaneous detection and identification of Xanthomonas species causing tomato bacterial spot. Methods and Results: We verified the specificity and sensitivity of the previously reported sets of primers designed for strains of the four species of Brazilian tomato bacterial spot xanthomonads, consisting of 30 of Xanthomonas euvesicatoria, 30 of X. vesicatoria,50ofX. perforans and 50 of X. gardneri. Furthermore, we tested a multiplex PCR protocol for the purpose of concurrent species identification.
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| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Separatas biblioteca |
| Language: | English eng |
| Published: |
2012-09-27
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| Subjects: | Detecção, PCR, Bactéria, Doença, Identificação, Xanthomonas, |
| Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/934722 |
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| Summary: | To establish protocols for the simultaneous detection and identification of Xanthomonas species causing tomato bacterial spot. Methods and Results: We verified the specificity and sensitivity of the previously reported sets of primers designed for strains of the four species of Brazilian tomato bacterial spot xanthomonads, consisting of 30 of Xanthomonas euvesicatoria, 30 of X. vesicatoria,50ofX. perforans and 50 of X. gardneri. Furthermore, we tested a multiplex PCR protocol for the purpose of concurrent species identification. |
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