Molecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29A:AtDREB1A for the improvement of drought tolerance.

We evaluated the molecular, anatomical and physiological properties of a soybean line transformed to improve drought tolerance with an rd29A:AtDREB1A construct. This construct expressed dehydration- responsive element binding protein DREB1A from the stress-inducible rd29A promoter. The greenhouse growth test included four randomized blocks of soybean plants, with each treatment performed in triplicate. Seeds from the non-transformed soybean cultivar BR16 and from the genetically modified soybean P58 line (T2 generation) were grown at 15% gravimetric humidity for 31 days. To induce water deficit, the humidity was reduced to 5% gravimetric humidity (moderate stress) for 29 days and then to 2.5% gravimetric humidity (severe stress). AtDREB1A gene expression was higher in the genetically modified P58 plants during water deficit, demonstrating transgene stability in T2 generations and induction of the rd29A promoter. Drought-response genes, including GmPI-PLC, GmSTP, GmGRP, and GmLEA14, were highly expressed in plants submitted to severe stress. Genetically modified plants had higher stomatal conductance and consequently higher photosynthetic and transpiration rates. In addition, they had more chlorophyll. Overexpression of AtDREB1A may contribute to a decrease in leaf thickness; however, a thicker abaxial epidermis was observed. Overexpression of AtDREB1A in soybean appears to enhance drought tolerance.

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Main Authors: POLIZEL, A. M., MEDRI, M. E., NAKASHIMA, K., YAMANAKA, N., FARIAS, J. R. B., OLIVEIRA, M. C. N. de, MARIN, S. R. R., ABDELNOOR, R. V., MARCELINO, F. C., FUGANTI, R., RODRIGUES, F. A., STOLF-MOREIRA, R., BENEVENTI, M. A., ROLLA, A. A. P., NEUMAIER, N., YAMAGUCHI-SHINOZAKI, K., CARVALHO, J. F. C., NEPOMUCENO, A. L.
Other Authors: UEL; UEL; JIRCAS; JIRCAS; JOSE RENATO BOUCAS FARIAS, CNPSO; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; SILVANA REGINA ROCKENBACH MARIN, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; FRANCISMAR CORREA MARCELINO, CNPSO; RENATA FUGANTI, CNPSo; CNPSo; RENATA STOLF MOREIRA, CNPSo; UFRGS; AMANDA A. P. ROLLA, UEL; NORMAN NEUMAIER, CNPSO; JIRCAS; CNPSo; ALEXANDRE LIMA NEPOMUCENO, SRI.
Format: Separatas biblioteca
Language:English
eng
Published: 2012-02-17
Subjects:Soja, Resistência a seca, Fisiologia vegetal, Soybeans, Drought tolerance, Plant physiology,
Online Access:http://www.alice.cnptia.embrapa.br/alice/handle/doc/916042
http://dx.doi.org/10.4238/2011.October.21.4
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spelling dig-alice-doc-9160422017-08-16T04:36:18Z Molecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29A:AtDREB1A for the improvement of drought tolerance. POLIZEL, A. M. MEDRI, M. E. NAKASHIMA, K. YAMANAKA, N. FARIAS, J. R. B. OLIVEIRA, M. C. N. de MARIN, S. R. R. ABDELNOOR, R. V. MARCELINO, F. C. FUGANTI, R. RODRIGUES, F. A. STOLF-MOREIRA, R. BENEVENTI, M. A. ROLLA, A. A. P. NEUMAIER, N. YAMAGUCHI-SHINOZAKI, K. CARVALHO, J. F. C. NEPOMUCENO, A. L. UEL; UEL; JIRCAS; JIRCAS; JOSE RENATO BOUCAS FARIAS, CNPSO; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; SILVANA REGINA ROCKENBACH MARIN, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; FRANCISMAR CORREA MARCELINO, CNPSO; RENATA FUGANTI, CNPSo; CNPSo; RENATA STOLF MOREIRA, CNPSo; UFRGS; AMANDA A. P. ROLLA, UEL; NORMAN NEUMAIER, CNPSO; JIRCAS; CNPSo; ALEXANDRE LIMA NEPOMUCENO, SRI. Soja Resistência a seca Fisiologia vegetal Soybeans Drought tolerance Plant physiology We evaluated the molecular, anatomical and physiological properties of a soybean line transformed to improve drought tolerance with an rd29A:AtDREB1A construct. This construct expressed dehydration- responsive element binding protein DREB1A from the stress-inducible rd29A promoter. The greenhouse growth test included four randomized blocks of soybean plants, with each treatment performed in triplicate. Seeds from the non-transformed soybean cultivar BR16 and from the genetically modified soybean P58 line (T2 generation) were grown at 15% gravimetric humidity for 31 days. To induce water deficit, the humidity was reduced to 5% gravimetric humidity (moderate stress) for 29 days and then to 2.5% gravimetric humidity (severe stress). AtDREB1A gene expression was higher in the genetically modified P58 plants during water deficit, demonstrating transgene stability in T2 generations and induction of the rd29A promoter. Drought-response genes, including GmPI-PLC, GmSTP, GmGRP, and GmLEA14, were highly expressed in plants submitted to severe stress. Genetically modified plants had higher stomatal conductance and consequently higher photosynthetic and transpiration rates. In addition, they had more chlorophyll. Overexpression of AtDREB1A may contribute to a decrease in leaf thickness; however, a thicker abaxial epidermis was observed. Overexpression of AtDREB1A in soybean appears to enhance drought tolerance. 2017-07-13T18:48:17Z 2017-07-13T18:48:17Z 2012-02-17 2011 2017-07-13T18:48:17Z Separatas Genetics and Molecular Research, Ribeirão Preto, v. 10, n. 4, p. 3641-3656, 2011. http://www.alice.cnptia.embrapa.br/alice/handle/doc/916042 http://dx.doi.org/10.4238/2011.October.21.4 en eng openAccess
institution EMBRAPA
collection DSpace
country Brasil
countrycode BR
component Bibliográfico
access En linea
databasecode dig-alice
tag biblioteca
region America del Sur
libraryname Sistema de bibliotecas de EMBRAPA
language English
eng
topic Soja
Resistência a seca
Fisiologia vegetal
Soybeans
Drought tolerance
Plant physiology
Soja
Resistência a seca
Fisiologia vegetal
Soybeans
Drought tolerance
Plant physiology
spellingShingle Soja
Resistência a seca
Fisiologia vegetal
Soybeans
Drought tolerance
Plant physiology
Soja
Resistência a seca
Fisiologia vegetal
Soybeans
Drought tolerance
Plant physiology
POLIZEL, A. M.
MEDRI, M. E.
NAKASHIMA, K.
YAMANAKA, N.
FARIAS, J. R. B.
OLIVEIRA, M. C. N. de
MARIN, S. R. R.
ABDELNOOR, R. V.
MARCELINO, F. C.
FUGANTI, R.
RODRIGUES, F. A.
STOLF-MOREIRA, R.
BENEVENTI, M. A.
ROLLA, A. A. P.
NEUMAIER, N.
YAMAGUCHI-SHINOZAKI, K.
CARVALHO, J. F. C.
NEPOMUCENO, A. L.
Molecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29A:AtDREB1A for the improvement of drought tolerance.
description We evaluated the molecular, anatomical and physiological properties of a soybean line transformed to improve drought tolerance with an rd29A:AtDREB1A construct. This construct expressed dehydration- responsive element binding protein DREB1A from the stress-inducible rd29A promoter. The greenhouse growth test included four randomized blocks of soybean plants, with each treatment performed in triplicate. Seeds from the non-transformed soybean cultivar BR16 and from the genetically modified soybean P58 line (T2 generation) were grown at 15% gravimetric humidity for 31 days. To induce water deficit, the humidity was reduced to 5% gravimetric humidity (moderate stress) for 29 days and then to 2.5% gravimetric humidity (severe stress). AtDREB1A gene expression was higher in the genetically modified P58 plants during water deficit, demonstrating transgene stability in T2 generations and induction of the rd29A promoter. Drought-response genes, including GmPI-PLC, GmSTP, GmGRP, and GmLEA14, were highly expressed in plants submitted to severe stress. Genetically modified plants had higher stomatal conductance and consequently higher photosynthetic and transpiration rates. In addition, they had more chlorophyll. Overexpression of AtDREB1A may contribute to a decrease in leaf thickness; however, a thicker abaxial epidermis was observed. Overexpression of AtDREB1A in soybean appears to enhance drought tolerance.
author2 UEL; UEL; JIRCAS; JIRCAS; JOSE RENATO BOUCAS FARIAS, CNPSO; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; SILVANA REGINA ROCKENBACH MARIN, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; FRANCISMAR CORREA MARCELINO, CNPSO; RENATA FUGANTI, CNPSo; CNPSo; RENATA STOLF MOREIRA, CNPSo; UFRGS; AMANDA A. P. ROLLA, UEL; NORMAN NEUMAIER, CNPSO; JIRCAS; CNPSo; ALEXANDRE LIMA NEPOMUCENO, SRI.
author_facet UEL; UEL; JIRCAS; JIRCAS; JOSE RENATO BOUCAS FARIAS, CNPSO; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; SILVANA REGINA ROCKENBACH MARIN, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; FRANCISMAR CORREA MARCELINO, CNPSO; RENATA FUGANTI, CNPSo; CNPSo; RENATA STOLF MOREIRA, CNPSo; UFRGS; AMANDA A. P. ROLLA, UEL; NORMAN NEUMAIER, CNPSO; JIRCAS; CNPSo; ALEXANDRE LIMA NEPOMUCENO, SRI.
POLIZEL, A. M.
MEDRI, M. E.
NAKASHIMA, K.
YAMANAKA, N.
FARIAS, J. R. B.
OLIVEIRA, M. C. N. de
MARIN, S. R. R.
ABDELNOOR, R. V.
MARCELINO, F. C.
FUGANTI, R.
RODRIGUES, F. A.
STOLF-MOREIRA, R.
BENEVENTI, M. A.
ROLLA, A. A. P.
NEUMAIER, N.
YAMAGUCHI-SHINOZAKI, K.
CARVALHO, J. F. C.
NEPOMUCENO, A. L.
format Separatas
topic_facet Soja
Resistência a seca
Fisiologia vegetal
Soybeans
Drought tolerance
Plant physiology
author POLIZEL, A. M.
MEDRI, M. E.
NAKASHIMA, K.
YAMANAKA, N.
FARIAS, J. R. B.
OLIVEIRA, M. C. N. de
MARIN, S. R. R.
ABDELNOOR, R. V.
MARCELINO, F. C.
FUGANTI, R.
RODRIGUES, F. A.
STOLF-MOREIRA, R.
BENEVENTI, M. A.
ROLLA, A. A. P.
NEUMAIER, N.
YAMAGUCHI-SHINOZAKI, K.
CARVALHO, J. F. C.
NEPOMUCENO, A. L.
author_sort POLIZEL, A. M.
title Molecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29A:AtDREB1A for the improvement of drought tolerance.
title_short Molecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29A:AtDREB1A for the improvement of drought tolerance.
title_full Molecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29A:AtDREB1A for the improvement of drought tolerance.
title_fullStr Molecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29A:AtDREB1A for the improvement of drought tolerance.
title_full_unstemmed Molecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29A:AtDREB1A for the improvement of drought tolerance.
title_sort molecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29a:atdreb1a for the improvement of drought tolerance.
publishDate 2012-02-17
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/916042
http://dx.doi.org/10.4238/2011.October.21.4
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