Uso de sêmen resfriado e inseminação artificial em caprinos leiteiros na República de Cabo Verde.
Resumo: Resumo: Este projeto e fruto de um trabalho conjunto entre a Agencia Brasileira de Cooperacao Internacional, CNPq, Embrapa, Governo de Cabo Verde, GOPA, UFF e UFPR. Teve como objetivo treinar tecnicos caboverdianos, para que possam executar todas as etapas envolvidas em um programa de inseminacao artificial (IA); determinar um protocolo de IA com semen resfriado que possibilite a disseminacao de genetica para todas as ilhas do arquipelago; fazer uma primeira disseminacao genetica; estruturar um centro de coleta e manipulacao de semen caprino; alem de avaliar a viabilidade da utilizacao do semen caprino diluido em meio tris-gema 2,5% (Evans & Maxwell, 1987; modificado), resfriado a 5 ‹C e armazenado por diferentes periodos (24 ou 48 horas). Foram inseminadas por via transcervical 133 cabras sem raca definida e nativas da Republica de Cabo Verde, divididas aleatoriamente em dois tratamentos T24 e T48. O estro foi sincronizado com a utilizacao de esponjas intra-vaginais contendo 60 mg de acetato de medroxiprogesterona por seis dias; 37,5 ƒÊg de D-cloprostenol e 200 UI de eCG 24 horas antes da retirada da esponja. Foram utilizados tres reprodutores da Raca Canarias, foi utilizado dose inseminante de 150 x 106 de espermatozoides viaveis. Para resfriar e manter o semen a 5o C foi utilizado o BotutainerR (Biotech Botucatu, Reproducao Animal, Botucatu - SP) adaptado. As analises estatisticas foram realizadas utilizando-se o pacote computacional SAEG (2009). Nao houve diferenca (P>0,05) entre os padroes seminais para os diferentes periodos de resfriamento (T24 - 58,8% }11,1 de motilidade e 2,9 }0,5 de vigor; T48 - 51,3% }2,5 de motilidade e 2,8 }0,3 de vigor), o que permitiu obter taxas de paricao similares em ambos os tratamentos (T24-26,5% e T48-21,5%). A eficiencia dos protocolos testados permitiu a disseminacao de genetica caprina na Republica de Cabo Verde. Houve correlacao (r = 0,27; P<0,05) entre o intervalo de retirada do progestageno a inseminacao artificial (IRIA) com a profundidade de deposicao de semen (PROF). Houve tambem correlacao (r = 0,29; P<0,05) entre o IRIA e a taxa de paricao (PARI). Conclui-se que o semen caprino, resfriado por 48 horas a 50C, apresenta o mesmo potencial de fertilizacao do semen resfriado por 24 horas a 50C. Abstract: This project is the result of a collaborative effort among the Brazilian Agency for International Cooperation, the National Council of Scientific and Technological Development (CNPq), Brazilian Agricultural Research Corporation – Embrapa Goats and Sheep, the government of Cape Verde, GOPA (Worldwide Consultants), the Federal University of Rio de Janeiro (UFF) and the Federal University of Paraná (UFPR). It aimed to train Cape Verdeans technicians to perform all the steps involved in a program for goat artificial insemination (AI); to determine a protocol for AI with chilled semen that enables the dissemination of genetics to all islands of the archipelago; to make a first genetic spread; and to structure a center for collection and handling goat semen. The fertilizing capacity of goat semen diluted in tris-egg yolk 2.5% (Evans & Maxwell, 1987; modified), chilled at 5 oC for 24 or 48 hours was evaluated. Transcervical artificial insemination was performed in 133 goats that were divided, randomly, into two treatments T24 and T48. The estrus was synchronized by the mean of intravaginal sponges containing 60 mg of medroxyprogesterone acetate for six days; 37.5 mg of D-cloprostenol and 200 IU eCG 24 hours before removing the sponge. Three Canarian’s Buck were used, the insemination dose was 150 x 106 mobile spermatozoa, for cooling and keeping the semen at 5 oC a Botutainer® (Biotech Botucatu, Animal Reproduction, Botucatu - SP) was adapted and used. There was no difference (P> 0.05) between the seminal patterns for the different periods of cooling (T24 - 58.8%±11.1 for motility and 2.9±0.5 for strength; T48 - 51.3%±2.5 for motility and 2.8±0.3 for strength), which allowed to obtain similar pregnancy rates in both treatments (T24 – 26.5% and T48 - 21,5%). The efficiency of the tested protocols allowed the dissemination of goat’s genetic material in the Republic of Cape Verde. There was a correlation (r = 0.27, P < 0.05) between the range of sponge withdrawal to artificial insemination (IRIA) with the depth of semen deposition (PROF). There was also a correlation (r = 0.29, P < 0.05) between IRIA and the calving rate (PARI). Statistical analysis was done in the computer package SAEG (2009). It was concluded that goat semen, cooled for 48 hours at 5 oC, has the same fertility that semen cooled for 24 hours at 5 oC.
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Format: | Teses biblioteca |
Language: | pt_BR por |
Published: |
2012-01-03
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Subjects: | Caprino, Reprodução animal, Inseminação artificial, Goats, Reproduction, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/911456 |
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Summary: | Resumo: Resumo: Este projeto e fruto de um trabalho conjunto entre a Agencia Brasileira de Cooperacao Internacional, CNPq, Embrapa, Governo de Cabo Verde, GOPA, UFF e UFPR. Teve como objetivo treinar tecnicos caboverdianos, para que possam executar todas as etapas envolvidas em um programa de inseminacao artificial (IA); determinar um protocolo de IA com semen resfriado que possibilite a disseminacao de genetica para todas as ilhas do arquipelago; fazer uma primeira disseminacao genetica; estruturar um centro de coleta e manipulacao de semen caprino; alem de avaliar a viabilidade da utilizacao do semen caprino diluido em meio tris-gema 2,5% (Evans & Maxwell, 1987; modificado), resfriado a 5 ‹C e armazenado por diferentes periodos (24 ou 48 horas). Foram inseminadas por via transcervical 133 cabras sem raca definida e nativas da Republica de Cabo Verde, divididas aleatoriamente em dois tratamentos T24 e T48. O estro foi sincronizado com a utilizacao de esponjas intra-vaginais contendo 60 mg de acetato de medroxiprogesterona por seis dias; 37,5 ƒÊg de D-cloprostenol e 200 UI de eCG 24 horas antes da retirada da esponja. Foram utilizados tres reprodutores da Raca Canarias, foi utilizado dose inseminante de 150 x 106 de espermatozoides viaveis. Para resfriar e manter o semen a 5o C foi utilizado o BotutainerR (Biotech Botucatu, Reproducao Animal, Botucatu - SP) adaptado. As analises estatisticas foram realizadas utilizando-se o pacote computacional SAEG (2009). Nao houve diferenca (P>0,05) entre os padroes seminais para os diferentes periodos de resfriamento (T24 - 58,8% }11,1 de motilidade e 2,9 }0,5 de vigor; T48 - 51,3% }2,5 de motilidade e 2,8 }0,3 de vigor), o que permitiu obter taxas de paricao similares em ambos os tratamentos (T24-26,5% e T48-21,5%). A eficiencia dos protocolos testados permitiu a disseminacao de genetica caprina na Republica de Cabo Verde. Houve correlacao (r = 0,27; P<0,05) entre o intervalo de retirada do progestageno a inseminacao artificial (IRIA) com a profundidade de deposicao de semen (PROF). Houve tambem correlacao (r = 0,29; P<0,05) entre o IRIA e a taxa de paricao (PARI). Conclui-se que o semen caprino, resfriado por 48 horas a 50C, apresenta o mesmo potencial de fertilizacao do semen resfriado por 24 horas a 50C. Abstract: This project is the result of a collaborative effort among the Brazilian Agency for International Cooperation, the National Council of Scientific and Technological Development (CNPq), Brazilian Agricultural Research Corporation – Embrapa Goats and Sheep, the government of Cape Verde, GOPA (Worldwide Consultants), the Federal University of Rio de Janeiro (UFF) and the Federal University of Paraná (UFPR). It aimed to train Cape Verdeans technicians to perform all the steps involved in a program for goat artificial insemination (AI); to determine a protocol for AI with chilled semen that enables the dissemination of genetics to all islands of the archipelago; to make a first genetic spread; and to structure a center for collection and handling goat semen. The fertilizing capacity of goat semen diluted in tris-egg yolk 2.5% (Evans & Maxwell, 1987; modified), chilled at 5 oC for 24 or 48 hours was evaluated. Transcervical artificial insemination was performed in 133 goats that were divided, randomly, into two treatments T24 and T48. The estrus was synchronized by the mean of intravaginal sponges containing 60 mg of medroxyprogesterone acetate for six days; 37.5 mg of D-cloprostenol and 200 IU eCG 24 hours before removing the sponge. Three Canarian’s Buck were used, the insemination dose was 150 x 106 mobile spermatozoa, for cooling and keeping the semen at 5 oC a Botutainer® (Biotech Botucatu, Animal Reproduction, Botucatu - SP) was adapted and used. There was no difference (P> 0.05) between the seminal patterns for the different periods of cooling (T24 - 58.8%±11.1 for motility and 2.9±0.5 for strength; T48 - 51.3%±2.5 for motility and 2.8±0.3 for strength), which allowed to obtain similar pregnancy rates in both treatments (T24 – 26.5% and T48 - 21,5%). The efficiency of the tested protocols allowed the dissemination of goat’s genetic material in the Republic of Cape Verde. There was a correlation (r = 0.27, P < 0.05) between the range of sponge withdrawal to artificial insemination (IRIA) with the depth of semen deposition (PROF). There was also a correlation (r = 0.29, P < 0.05) between IRIA and the calving rate (PARI). Statistical analysis was done in the computer package SAEG (2009). It was concluded that goat semen, cooled for 48 hours at 5 oC, has the same fertility that semen cooled for 24 hours at 5 oC. |
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