Anaplasma marginale infection in cattle from southwestern Amazonia.

study provides the first epidemiological data regarding infection by Anaplasma marginale in cattle reared in south-western Brazilian Amazonia. One simple procedure was adapted for the extraction of DNA from blood clots collected in seven microregions of Rondônia State and two mesoregions of Acre State. PCR method was used to asses the frequency of A. marginale infections in 4 to12-month-old cattle. The cattle infection was investigated by polymerase chain reaction (PCR) using the specific primer ?msp5? for A. marginale. The DNA amplifications revealed that the mean frequency of A. marginale infection was 98.6% (1,627/1,650) in samples from Rondonia, and 92.87% (208/225) in samples from Acre. The high frequency of A. marginale infections in 4 to 12- month-old cattle indicate a situation of enzootic stability in the studied areas and are comparable to those detected by immunodiagnosis in different endemic regions in Brazil. The DNA extraction of clotted blood method described here can be used for epidemiological studies on anaplasmosis and other bovine hemoparasites.

Bibliographic Details

Main Authors:	BRITO, L. G., OLIVEIRA, M. C. de S., ROCHA, R. B., SILVA NETTO, F. G. da, MARIM, A. D., SOUZA, G. C. R. de, VENDRAME, F. B., MOURA, M. M. da F.
Other Authors:	LUCIANA GATTO BRITO, CPAF-RO; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; RODRIGO BARROS ROCHA, CPAF-RO; FRANCELINO GOULART DA SILVA NETTO, EMBRAPA RONDÔNIA; ADRIANA DENISE MARIM, UNIVERSIDADE FEDERAL DE RONDÔNIA; GISLANE CRISTINA RODRIGUES DE SOUZA, UNIVERSIDADE FEDERAL DE RONDÔNIA; FABIANO BENITEZ VENDRAME, AGENCIA DE DEFESA SANITÁRIA AGROSILVOPASTORIL; MARIA MANUELA DA FONSECA MOURA, UNIVERSIDADE FEDERAL DE RONDÔNIA/ PORTO VELHO.
Format:	Separatas biblioteca
Language:	pt_BR por
Published:	2010-12-08
Subjects:	Cattle south., Anaplasma Marginale., epidemiology.,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/869118
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