Genetic maps of Saccharum officinarum L. and Saccharum robustum Brandes & Jew ex grassl.
Genetic analysis was performed in a population composed of 100 F1 individuals derived from a cross between a cultivated sugarcane (S. officinarum La Purple) and its proposed progenitor species (S. robustum "Mol 5829'). Various types (arbitrarily primed-PCR, RFLPs, and AFLPs) of single-dose DNA markers (SDMs) were used to construct genetic linkage maps to both species. The LA Purple map was composed of 341 SDMs, spanning 74 linkage groups and 1.881 cM, white the Mol 5829 map contained 301 SDMs, spanning 65 linkage gropus and 1,189 cM, Transmission genetics in these two species showed incomplete polysomy based on the detection of 15% of SDMs linked in repulsion in LA Purple and 13% of these in Mol 5829. Because of this incomplete polysomy, multiple-dose markers could not be mapped for lack of a genetic model for their segregation. Due to inclusion of RFLP anchor probes, conserved in related species, the resulting maps will serve as useful tools for breeding, ecology, evolution, and molecular biology studies within the Andropogoneae.
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Parte de livro biblioteca |
| Language: | pt_BR por |
| Published: |
2000-03-14
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| Subjects: | Mapeamento, Mapping, Biotehnology., Biotecnologia, Cana de Açúcar, DNA, Genética., genetics, sugarcane., |
| Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/483496 |
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| Summary: | Genetic analysis was performed in a population composed of 100 F1 individuals derived from a cross between a cultivated sugarcane (S. officinarum La Purple) and its proposed progenitor species (S. robustum "Mol 5829'). Various types (arbitrarily primed-PCR, RFLPs, and AFLPs) of single-dose DNA markers (SDMs) were used to construct genetic linkage maps to both species. The LA Purple map was composed of 341 SDMs, spanning 74 linkage groups and 1.881 cM, white the Mol 5829 map contained 301 SDMs, spanning 65 linkage gropus and 1,189 cM, Transmission genetics in these two species showed incomplete polysomy based on the detection of 15% of SDMs linked in repulsion in LA Purple and 13% of these in Mol 5829. Because of this incomplete polysomy, multiple-dose markers could not be mapped for lack of a genetic model for their segregation. Due to inclusion of RFLP anchor probes, conserved in related species, the resulting maps will serve as useful tools for breeding, ecology, evolution, and molecular biology studies within the Andropogoneae. |
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