Evaluating the microbial diversity of soil samples: methodological innovations.
This manuscript is a review of the innovative methodlogies that enable more precise evaluation of soil microbial diversity. Highlighting the molecular approach, which does not require the isolation of microorganisms and allows the inclusion of non-culturable genotypes in the analyses, the described methodologies revolutionised the environmental microbiology and opened gateways for an accurate understanding of the ecology and diversity of microorganisms. The application of techniques based on soil total DNA extraction, PCR amplification of genes or gene fragments, and sequence analysis revealed that the microbial universe in far more complex than ever imagined. Examples of applications of the molecular approach to study the diversity of soil diazotrophic bacteria are given.
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Artigo de periódico biblioteca |
| Language: | Ingles English |
| Published: |
2002-11-22
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| Subjects: | Microbial diversity, DGGE, Molecular methods, Soil DNA, |
| Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/336250 |
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| Summary: | This manuscript is a review of the innovative methodlogies that enable more precise evaluation of soil microbial diversity. Highlighting the molecular approach, which does not require the isolation of microorganisms and allows the inclusion of non-culturable genotypes in the analyses, the described methodologies revolutionised the environmental microbiology and opened gateways for an accurate understanding of the ecology and diversity of microorganisms. The application of techniques based on soil total DNA extraction, PCR amplification of genes or gene fragments, and sequence analysis revealed that the microbial universe in far more complex than ever imagined. Examples of applications of the molecular approach to study the diversity of soil diazotrophic bacteria are given. |
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