Does sorghum phenolic extract have antifungal effect?
This study aimed to evaluate the antifungal efect of SC319 sorghum phenolic extract (SPE) on the Aspergillus, Fusarium, Penicillium, Stenocarpella, Colletotrichum, and Macrophomina genera. SPE was extracted by 20% ethanol and used in four assays: (1) against Fusarium verticillioides in solid (PDA) and liquid (PD) potato dextrose media; (2) Minimum Inhibitory Concentration (MIC) assay with 16 fungi isolates; (3) Conidial Germination Rate (CGR) with 14 fungi isolates and (4) Growth Curve (GC) with 11 fungi isolates. There was no reduction in the mycelial growth (colony diameter and dry weight) and in the number of Fusarium verticillioides spores in assay 1 (PDA and PD). The colony's dry weight was almost six times higher in the presence than in the absence of SPE. All SPE samples presented MIC (assay 1) above the maximum concentration tested (5000 µg.mL−1) for the 16 isolates. Also, there was no inhibitory efect of SPE on conidia germination rate (CGR). Oppositely, in GC assay, the control had a higher CFU count than the samples with SPE in 24 h. This result suggests that SPE can delay the fungal growth in the frst hours of incubation, which is an important fnding that may help reduce the severity of fungal diseases in plants. However, further studies are needed to confrm these results, including sorghum genotypes with diferent profles of phenolic compounds. Although the SC319 SPE was not efective as an antifungal agent, it may have potential as a growth promoter of benefcial fungi in the food and pharmaceutical industries.
| Main Authors: | , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Artigo de periódico biblioteca |
| Language: | eng |
| Published: |
2024-05-10
|
| Subjects: | Concentração inibitória mínima, Biofungicida, Fungo toxigênico, Toxigenic fungi, Biofungicide, Sorgo, Sorghum Bicolor, Minimum inhibitory concentration, |
| Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1164179 https://doi.org/10.1007/s42770-024-01327-9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | This study aimed to evaluate the antifungal efect of SC319 sorghum phenolic extract (SPE) on the Aspergillus, Fusarium, Penicillium, Stenocarpella, Colletotrichum, and Macrophomina genera. SPE was extracted by 20% ethanol and used in four assays: (1) against Fusarium verticillioides in solid (PDA) and liquid (PD) potato dextrose media; (2) Minimum Inhibitory Concentration (MIC) assay with 16 fungi isolates; (3) Conidial Germination Rate (CGR) with 14 fungi isolates and (4) Growth Curve (GC) with 11 fungi isolates. There was no reduction in the mycelial growth (colony diameter and dry weight) and in the number of Fusarium verticillioides spores in assay 1 (PDA and PD). The colony's dry weight was almost six times higher in the presence than in the absence of SPE. All SPE samples presented MIC (assay 1) above the maximum concentration tested (5000 µg.mL−1) for the 16 isolates. Also, there was no inhibitory efect of SPE on conidia germination rate (CGR). Oppositely, in GC assay, the control had a higher CFU count than the samples with SPE in 24 h. This result suggests that SPE can delay the fungal growth in the frst hours of incubation, which is an important fnding that may help reduce the severity of fungal diseases in plants. However, further studies are needed to confrm these results, including sorghum genotypes with diferent profles of phenolic compounds. Although the SC319 SPE was not efective as an antifungal agent, it may have potential as a growth promoter of benefcial fungi in the food and pharmaceutical industries. |
|---|