Efficiency of coffee seeds rna extraction protoco using rna integrity number analysis.
Coffee is one of the most important agricultural products in the world market. The mode of coffee processing, whether wet or dry, determines the flavor characteristic and also the seed quality. Quality RNA isolation is a mandatory requirement for studies of gene expression, including reverse transcriptase (RT), real-time quantitative PCR (RT-qPCR), construction of cDNA libraries, or microarray analyses. Due to the presence of secondary metabolites, polysaccharides, and polyphenols, standardization of a quality RNA extraction for different coffee plant tissue is very difficult. Also, getting high-quality RNA may be complicated because of RNA susceptibility to RNase degradation.
Saved in:
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | Anais e Proceedings de eventos biblioteca |
| Language: | Portugues pt_BR |
| Published: |
2020-11-18
|
| Subjects: | Extração, RNA, Semente, Café, |
| Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126783 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Coffee is one of the most important agricultural products in the world market. The mode of coffee processing, whether wet or dry, determines the flavor characteristic and also the seed quality. Quality RNA isolation is a mandatory requirement for studies of gene expression, including reverse transcriptase (RT), real-time quantitative PCR (RT-qPCR), construction of cDNA libraries, or microarray analyses. Due to the presence of secondary metabolites, polysaccharides, and polyphenols, standardization of a quality RNA extraction for different coffee plant tissue is very difficult. Also, getting high-quality RNA may be complicated because of RNA susceptibility to RNase degradation. |
|---|