MALDI-TOF mass spectrometry identification and detection of relevant pathogens in beef cattle.

MALDI-TOF mass spectrometry identification and detection of relevant pathogens in beef cattle.

Mycobacterium bovis, Brucella abortus and Salmonella species account for significant economic losses in cattle production worldwide, besides being public health threats. We have established MALDI-TOF (Matrix Assisted Laser Desorption Ionization ? Time-of-Flight) mass spectrometry with modified protein extraction methods and improved the presently available reference spectra libraries, in order to effectively detect those pathogens from bovine samples.

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Bibliographic Details
Main Authors: BACANELLI, G., MONTOVANI, C., LOUZAN, A. L., PASQUATTI, T., BIER, D., ROSINHA, G. M. S., ARAUJO, F. R., VERBISCK, N. V.
Other Authors: Gisele Bacanelli, Universidade Federal de Mato Grosso do Sul - UFMS; Cynthia Mantovani, Universidade Federal de Mato Grosso do Sul - UFMS; Anna Letícia Louzan, Universidade Federal de Mato Grosso do Sul - UFMS; Taynara Pasquatti, Universidade Católica Dom Bosco - UCDB; Daniele Bier, Universidade Católica Dom Bosco - UCDB; GRACIA MARIA SOARES ROSINHA, CNPGC; FLABIO RIBEIRO DE ARAUJO, CNPGC; NEWTON VALERIO VERBISCK, CNPGC.
Format: Anais e Proceedings de eventos biblioteca
Language:English
eng
Published: 2018-12-27
Subjects:Salmonella, Brucella, Mycobacterium, Matrix-assisted laser desorption-ionization mass, Beef cattle,
Online Access:http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102578
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Summary:Mycobacterium bovis, Brucella abortus and Salmonella species account for significant economic losses in cattle production worldwide, besides being public health threats. We have established MALDI-TOF (Matrix Assisted Laser Desorption Ionization ? Time-of-Flight) mass spectrometry with modified protein extraction methods and improved the presently available reference spectra libraries, in order to effectively detect those pathogens from bovine samples.

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