Evaluation of nested and semi nested PCR in detection of equine infectious anemia virus infection in horses of Pantanal region, Brazil.
Equine infectious anemia (EIA) is caused by a retrovirus and its primary mode of transmission comprises transfer of blood from infected animals to healthy ones. In Brazil, EIA notification is mandatory and its confirmation is based in serological tests followed by euthanasia of infected animals. AGID and ELISA are serological techniques recommended because have good accuracy and reliable results for detection of positive animals; however, some cases may display false results. Based on that, the aim of this study was to evaluate nested PCR technique in samples from Brazil. We tested five combinations of these primers, using 12 DNA samples extracted from positive horses from Pantanal (endemic area) in both serological techniques.
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| Main Authors: | , , |
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| Other Authors: | |
| Format: | Separatas biblioteca |
| Language: | English eng |
| Published: |
2017-09-27
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| Subjects: | Equino, EIAV (Equine infectious anemia virus)., Anemia Infecciosa Equina, Virus., Epidemiology, Equine infectious anemia, Horse diseases., |
| Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076399 |
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| Summary: | Equine infectious anemia (EIA) is caused by a retrovirus and its primary mode of transmission comprises transfer of blood from infected animals to healthy ones. In Brazil, EIA notification is mandatory and its confirmation is based in serological tests followed by euthanasia of infected animals. AGID and ELISA are serological techniques recommended because have good accuracy and reliable results for detection of positive animals; however, some cases may display false results. Based on that, the aim of this study was to evaluate nested PCR technique in samples from Brazil. We tested five combinations of these primers, using 12 DNA samples extracted from positive horses from Pantanal (endemic area) in both serological techniques. |
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