Cryotolerance and pregnancy rates after exposure of bovine in vitro-produced embryos to forskolin and linoleic acid before vitrification.
The objective of the present study was to evaluate the effects of supplementation of in vitro culture (IVC) medium with drugs that stimulates the lipolysis (Forskolin: Forsk) and inhibit the lipogenesis (Linoleic Acid LA) on the intracytoplasmic lipid content and cryotolerance of bovine embryos (Experiment 1), as well as to evaluate the effect of treatment of embryos with Forsk on the pregnancy rates after transfer to synchronized recipients (Experiment 2).
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| Main Authors: | , , , , , , , , |
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| Format: | Separatas biblioteca |
| Language: | English eng |
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2016-10-20
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| Subjects: | Reprodução animal, Criopreservação, Cryopreservation, Forskolin, Vitrification, Animal reproduction, linoleic acid., |
| Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1055135 |
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dig-alice-doc-10551352017-08-16T03:47:46Z Cryotolerance and pregnancy rates after exposure of bovine in vitro-produced embryos to forskolin and linoleic acid before vitrification. MENEGHEL, M. DALL ACQUA, P. C. AMBROGI, M. GOUVÊA, L. M. ACHILLES, M. A. LEÃO, B. C. S. NOGUEIRA, E. ROCHA-FRIGONI, N. A. S. MINGOTI, G. Z. UNESP; UNESP; UNESP; Concepção Reprodução Bovina; Achilles Genetics - Biotecnologia Em Reprodução Animal; UNESP; ERIKLIS NOGUEIRA, CPAP; UNESP; UNESP. Reprodução animal Criopreservação Cryopreservation Forskolin Vitrification Animal reproduction linoleic acid. The objective of the present study was to evaluate the effects of supplementation of in vitro culture (IVC) medium with drugs that stimulates the lipolysis (Forskolin: Forsk) and inhibit the lipogenesis (Linoleic Acid LA) on the intracytoplasmic lipid content and cryotolerance of bovine embryos (Experiment 1), as well as to evaluate the effect of treatment of embryos with Forsk on the pregnancy rates after transfer to synchronized recipients (Experiment 2). Proceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain, September 9th and 10th, 2016. A327 Support Biotechnologies: Cryopreservation and cryobiology, diagnosis through imaging, molecular biology and ?omics?. Título em português: Criotolerância e taxa de concepção após exposição de embriões bovinos produzidos in vitro ao Forskolin ou ácido linoleico antes da vitrificação. 2016-10-20T11:11:11Z 2016-10-20T11:11:11Z 2016-10-20 2016 2017-01-18T11:11:11Z Separatas Animal Reproduction, v.13, n.3, p.701, Jul./Sept. 2016. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1055135 en eng openAccess |
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| topic |
Reprodução animal Criopreservação Cryopreservation Forskolin Vitrification Animal reproduction linoleic acid. Reprodução animal Criopreservação Cryopreservation Forskolin Vitrification Animal reproduction linoleic acid. |
| spellingShingle |
Reprodução animal Criopreservação Cryopreservation Forskolin Vitrification Animal reproduction linoleic acid. Reprodução animal Criopreservação Cryopreservation Forskolin Vitrification Animal reproduction linoleic acid. MENEGHEL, M. DALL ACQUA, P. C. AMBROGI, M. GOUVÊA, L. M. ACHILLES, M. A. LEÃO, B. C. S. NOGUEIRA, E. ROCHA-FRIGONI, N. A. S. MINGOTI, G. Z. Cryotolerance and pregnancy rates after exposure of bovine in vitro-produced embryos to forskolin and linoleic acid before vitrification. |
| description |
The objective of the present study was to evaluate the effects of supplementation of in vitro culture (IVC) medium with drugs that stimulates the lipolysis (Forskolin: Forsk) and inhibit the lipogenesis (Linoleic Acid LA) on the intracytoplasmic lipid content and cryotolerance of bovine embryos (Experiment 1), as well as to evaluate the effect of treatment of embryos with Forsk on the pregnancy rates after transfer to synchronized recipients (Experiment 2). |
| author2 |
UNESP; UNESP; UNESP; Concepção Reprodução Bovina; Achilles Genetics - Biotecnologia Em Reprodução Animal; UNESP; ERIKLIS NOGUEIRA, CPAP; UNESP; UNESP. |
| author_facet |
UNESP; UNESP; UNESP; Concepção Reprodução Bovina; Achilles Genetics - Biotecnologia Em Reprodução Animal; UNESP; ERIKLIS NOGUEIRA, CPAP; UNESP; UNESP. MENEGHEL, M. DALL ACQUA, P. C. AMBROGI, M. GOUVÊA, L. M. ACHILLES, M. A. LEÃO, B. C. S. NOGUEIRA, E. ROCHA-FRIGONI, N. A. S. MINGOTI, G. Z. |
| format |
Separatas |
| topic_facet |
Reprodução animal Criopreservação Cryopreservation Forskolin Vitrification Animal reproduction linoleic acid. |
| author |
MENEGHEL, M. DALL ACQUA, P. C. AMBROGI, M. GOUVÊA, L. M. ACHILLES, M. A. LEÃO, B. C. S. NOGUEIRA, E. ROCHA-FRIGONI, N. A. S. MINGOTI, G. Z. |
| author_sort |
MENEGHEL, M. |
| title |
Cryotolerance and pregnancy rates after exposure of bovine in vitro-produced embryos to forskolin and linoleic acid before vitrification. |
| title_short |
Cryotolerance and pregnancy rates after exposure of bovine in vitro-produced embryos to forskolin and linoleic acid before vitrification. |
| title_full |
Cryotolerance and pregnancy rates after exposure of bovine in vitro-produced embryos to forskolin and linoleic acid before vitrification. |
| title_fullStr |
Cryotolerance and pregnancy rates after exposure of bovine in vitro-produced embryos to forskolin and linoleic acid before vitrification. |
| title_full_unstemmed |
Cryotolerance and pregnancy rates after exposure of bovine in vitro-produced embryos to forskolin and linoleic acid before vitrification. |
| title_sort |
cryotolerance and pregnancy rates after exposure of bovine in vitro-produced embryos to forskolin and linoleic acid before vitrification. |
| publishDate |
2016-10-20 |
| url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1055135 |
| work_keys_str_mv |
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