Cryotolerance and pregnancy rates after exposure of bovine in vitro-produced embryos to forskolin and linoleic acid before vitrification.

Cryotolerance and pregnancy rates after exposure of bovine in vitro-produced embryos to forskolin and linoleic acid before vitrification.

The objective of the present study was to evaluate the effects of supplementation of in vitro culture (IVC) medium with drugs that stimulates the lipolysis (Forskolin: Forsk) and inhibit the lipogenesis (Linoleic Acid LA) on the intracytoplasmic lipid content and cryotolerance of bovine embryos (Experiment 1), as well as to evaluate the effect of treatment of embryos with Forsk on the pregnancy rates after transfer to synchronized recipients (Experiment 2).

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Bibliographic Details
Main Authors: MENEGHEL, M., DALL ACQUA, P. C., AMBROGI, M., GOUVÊA, L. M., ACHILLES, M. A., LEÃO, B. C. S., NOGUEIRA, E., ROCHA-FRIGONI, N. A. S., MINGOTI, G. Z.
Other Authors: UNESP; UNESP; UNESP; Concepção Reprodução Bovina; Achilles Genetics - Biotecnologia Em Reprodução Animal; UNESP; ERIKLIS NOGUEIRA, CPAP; UNESP; UNESP.
Format: Separatas biblioteca
Language:English
eng
Published: 2016-10-20
Subjects:Reprodução animal, Criopreservação, Cryopreservation, Forskolin, Vitrification, Animal reproduction, linoleic acid.,
Online Access:http://www.alice.cnptia.embrapa.br/alice/handle/doc/1055135
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Summary:The objective of the present study was to evaluate the effects of supplementation of in vitro culture (IVC) medium with drugs that stimulates the lipolysis (Forskolin: Forsk) and inhibit the lipogenesis (Linoleic Acid LA) on the intracytoplasmic lipid content and cryotolerance of bovine embryos (Experiment 1), as well as to evaluate the effect of treatment of embryos with Forsk on the pregnancy rates after transfer to synchronized recipients (Experiment 2).

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