TRAP and SRAP markers to find genetic variability in complex polyploid Paullinia cupana var. sorbilis.

The guarana plant (Paullinia cupana var. sorbilis) is a polyploid rich in natural caffeine, used as a source for producing industrial soft drinks. Embrapa Western Amazon maintains an Active Germplasm Bank (BGA) with 270 clones, which represents the genetic basis of the species conservation and breeding programs. The BGA evaluations conducted using phenotypic traits and Random Amplified Polymorphic DNA (RAPD) markers indicated low genetic variability. Therefore, the objective of this study was to analyze the genetic diversity of the clonal germplasm of the guarana plant using Target Region Amplification Polymorphism (TRAP) and Sequence-Related Amplification Polymorphism (SRAP) markers.

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Bibliographic Details
Main Authors: SILVA, E. F. da, SOUSA, S. B. de, SILVA, G. F. da, SOUSA, N. R., NASCIMENTO FILHO, F. J. do, HANADA, R. E.
Other Authors: Elizangela Farias da Silva; Sandra Barbosa de Sousa; GILVAN FERREIRA DA SILVA, CPAA; NELCIMAR REIS SOUSA, CPACP; FIRMINO JOSE DO NASCIMENTO FILHO, CPAA; Rogério Eiji Hanada, INPA.
Format: Separatas biblioteca
Language:English
eng
Published: 2016-04-05
Subjects:Guarana plant, Clonal Cultivar, Amazon germplasm, Molecular marker., Guaraná, Germoplasma, Marcador Molecular, Paullinia Cupana, Variedade., germplasm.,
Online Access:http://www.alice.cnptia.embrapa.br/alice/handle/doc/1042648
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Summary:The guarana plant (Paullinia cupana var. sorbilis) is a polyploid rich in natural caffeine, used as a source for producing industrial soft drinks. Embrapa Western Amazon maintains an Active Germplasm Bank (BGA) with 270 clones, which represents the genetic basis of the species conservation and breeding programs. The BGA evaluations conducted using phenotypic traits and Random Amplified Polymorphic DNA (RAPD) markers indicated low genetic variability. Therefore, the objective of this study was to analyze the genetic diversity of the clonal germplasm of the guarana plant using Target Region Amplification Polymorphism (TRAP) and Sequence-Related Amplification Polymorphism (SRAP) markers.