TRAP and SRAP markers to find genetic variability in complex polyploid Paullinia cupana var. sorbilis.
The guarana plant (Paullinia cupana var. sorbilis) is a polyploid rich in natural caffeine, used as a source for producing industrial soft drinks. Embrapa Western Amazon maintains an Active Germplasm Bank (BGA) with 270 clones, which represents the genetic basis of the species conservation and breeding programs. The BGA evaluations conducted using phenotypic traits and Random Amplified Polymorphic DNA (RAPD) markers indicated low genetic variability. Therefore, the objective of this study was to analyze the genetic diversity of the clonal germplasm of the guarana plant using Target Region Amplification Polymorphism (TRAP) and Sequence-Related Amplification Polymorphism (SRAP) markers.
Main Authors: | , , , , , |
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Other Authors: | |
Format: | Separatas biblioteca |
Language: | English eng |
Published: |
2016-04-05
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Subjects: | Guarana plant, Clonal Cultivar, Amazon germplasm, Molecular marker., Guaraná, Germoplasma, Marcador Molecular, Paullinia Cupana, Variedade., germplasm., |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1042648 |
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Summary: | The guarana plant (Paullinia cupana var. sorbilis) is a polyploid rich in natural caffeine, used as a source for producing industrial soft drinks. Embrapa Western Amazon maintains an Active Germplasm Bank (BGA) with 270 clones, which represents the genetic basis of the species conservation and breeding programs. The BGA evaluations conducted using phenotypic traits and Random Amplified Polymorphic DNA (RAPD) markers indicated low genetic variability. Therefore, the objective of this study was to analyze the genetic diversity of the clonal germplasm of the guarana plant using Target Region Amplification Polymorphism (TRAP) and Sequence-Related Amplification Polymorphism (SRAP) markers. |
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