A simple and highly efficient Agrobacterium-mediated transformation protocol for Setaria viridis.

Abstract: The production and use of sugarcane in Brazil is very important for bioenergy production and is recognized as one of the most efficient in the world. In our laboratory, Setaria viridis is being tested as a model plant for sugarcane. S. viridis has biological attributes (rapid life cycle, small genome, diploid, short stature and simple growth requirements) that make it suitable for use as a model system. We report a highly efficient protocol for Agrobacterium-mediated genetic transformation of S. viridis. The optimization of several steps in tissue culture allowed the rapid regeneration of plants and increased the rate of transformation up to 29%. This protocol could become a powerful tool for functional genomics in sugarcane

Bibliographic Details

Main Authors:	MARTINS, P. K., RIBEIRO, A. P., DIAS, B. B. A., KOBAYASHI, A. K., MOLINARI, H. B. C.
Other Authors:	Polyana Kelly Martins; Ana Paula Ribeiro; BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; ADILSON KENJI KOBAYASHI, CNPAE; HUGO BRUNO CORREA MOLINARI, CNPAE.
Format:	Artigo de periódico biblioteca
Language:	Ingles English
Published:	2015-09-30
Subjects:	Model system, C4 metabolism, Green millet, Cana de Açúcar, Bioenergia, Milho Verde, Genoma, Bioenergy, Sugarcane, Setaria viridis, Genomics,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1025487 https://doi.org/10.1016/j.btre.2015.02.002
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