Unravelling the trans-kingdom function of Sstrf2, tRNA-derived small RNA of Sclerotinia sclerotiorum
In Sclerotinia sclerotiorum, white mold fungus, tRNA-derived small RNAs (tRFs) potentially belong to a new class of virulence effector because we previously reported two very abundant of larger size at 28-30 nt. In this study, we show that the tRFs have no fungal targets predicted based on transcriptome, degradome and small RNA-Seq of S. sclerotiorum. The tRFs function in a trans-kingdom manner was also evidenced by immunoprecipitation of argonaute protein 1 (Ago1) profiled by small RNA-Seq, as well as A. thaliana ago1 knockout mutant is more tolerant to DK3 inoculation.
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| Main Author: | |
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| Format: | Dataset biblioteca |
| Published: |
2021
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| Subjects: | Genetics, raw sequence reads, |
| Online Access: | https://figshare.com/articles/dataset/Unravelling_the_trans-kingdom_function_of_Sstrf2_tRNA-derived_small_RNA_of_Sclerotinia_sclerotiorum/25088831 |
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| Summary: | In Sclerotinia sclerotiorum, white mold fungus, tRNA-derived small RNAs (tRFs) potentially belong to a new class of virulence effector because we previously reported two very abundant of larger size at 28-30 nt. In this study, we show that the tRFs have no fungal targets predicted based on transcriptome, degradome and small RNA-Seq of S. sclerotiorum. The tRFs function in a trans-kingdom manner was also evidenced by immunoprecipitation of argonaute protein 1 (Ago1) profiled by small RNA-Seq, as well as A. thaliana ago1 knockout mutant is more tolerant to DK3 inoculation. |
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