Data from: Gene expression during the early stages of host perception and attachment in adult female Rhipicephalus microplus ticks

<p>This study produced a comprehensive transcriptome from newly molted adult ticks and will provide a useful resource for studies of tick feeding and host perception and also assist genome annotation refinements.</p> <p>The goal was to develop a comprehensive transcriptome of adult female Rhipicephalus microplus ticks and discover transcriptome changes that are related to feeding and host-perception prior to feeding. We have sequenced the transcriptomes of unfed newly molted adult female ticks, newly molted adult females that were feeding upon a bovine host for 3 hours following attachment to the host, and newly molted adult females that were placed in a gas-permeable bag attached to a bovine host for 3 hours (attachment to host prevented but host odors available to the ticks). There were 2 biological replicates and transcriptomes assembled from each individual experimental condition. A collective adult female transcriptome was assembled from sequence data pooled from all 3 conditions (using data from all replicates). Differential gene expression was examined using all possible comparisons to detect putative feeding-associated and host perception-associated transcripts. </p><div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Gene expression during the early stages of host perception and attachment in adult female Rhipicephalus microplus ticks.</p> <p>File Name: Web Page, url: <a href="https://doi.org/10.1007/s10493-019-00420-1">https://doi.org/10.1007/s10493-019-00420-1</a> </p></li></ul><p></p>

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Bibliographic Details
Main Authors: Kylie Bendele (17362513), Felix D. Guerrero (17477454), Deanna M. Bodine (17481423)
Format: Dataset biblioteca
Published: 2019
Subjects:Genomics and transcriptomics, Genetics, Gene expression (incl. microarray and other genome-wide approaches), cattle tick, feeding response, host perception, gene expression, transcriptomics, NP104, data.gov, ARS,
Online Access:https://figshare.com/articles/dataset/Data_from_Gene_expression_during_the_early_stages_of_host_perception_and_attachment_in_adult_female_Rhipicephalus_microplus_ticks/24853302
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Summary:<p>This study produced a comprehensive transcriptome from newly molted adult ticks and will provide a useful resource for studies of tick feeding and host perception and also assist genome annotation refinements.</p> <p>The goal was to develop a comprehensive transcriptome of adult female Rhipicephalus microplus ticks and discover transcriptome changes that are related to feeding and host-perception prior to feeding. We have sequenced the transcriptomes of unfed newly molted adult female ticks, newly molted adult females that were feeding upon a bovine host for 3 hours following attachment to the host, and newly molted adult females that were placed in a gas-permeable bag attached to a bovine host for 3 hours (attachment to host prevented but host odors available to the ticks). There were 2 biological replicates and transcriptomes assembled from each individual experimental condition. A collective adult female transcriptome was assembled from sequence data pooled from all 3 conditions (using data from all replicates). Differential gene expression was examined using all possible comparisons to detect putative feeding-associated and host perception-associated transcripts. </p><div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Gene expression during the early stages of host perception and attachment in adult female Rhipicephalus microplus ticks.</p> <p>File Name: Web Page, url: <a href="https://doi.org/10.1007/s10493-019-00420-1">https://doi.org/10.1007/s10493-019-00420-1</a> </p></li></ul><p></p>