Data from: Biological control of boxwood blight by Pseudomonas protegens recovered from recycling irrigation systems
<p>Boxwood (<em>Buxus</em> spp.) blight caused by <em>Calonectria pseudonaviculata</em> (<em>Cps</em>) is an emerging fungal disease in the United States. It has caused significant loss to the ornamental nursery and landscape industries. In this study, 1547 bacterial strains recovered from recycling irrigation systems were evaluated <em>in vitro</em> and <em>in planta</em> for their biological control efficacy against <em>Cps</em>. Three most potent strains 13A3, 14D5, and 34B6 identified all belonged to <em>Pseudomonas protegens</em>. Strains 13A3 and 14D5 reduced <em>Cps</em> mycelial growth by 95 to 100% in two sets of <em>in vitro</em> experiments including a 48-well plate assay and a dual culture assay. Strain 34B6 also reduced <em>Cps</em> growth by 93% in the 48-well plate assay and 66 to 72% in dual culture plates. <em>In planta</em>, strain 14D5 reduced <em>Cps</em> infection by more than 50%, while strains 13D3 and 34B6 by 40 to 46%. <em>Pseudomonas protegens</em> strains produced antifungal secondary metabolites that reduced <em>Cps</em> growth and infection. Other possible modes of action against boxwood blight are discussed. </p><div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Supplementary Table S1.</p> <p>File Name: Web Page, url: <a href="https://www.sciencedirect.com/science/article/pii/S1049964418300434?via=ihub#s0095">https://www.sciencedirect.com/science/article/pii/S1049964418300434?via=ihub#s0095</a> </p><p>Bacterial strains, the reduction of <em>Cps</em> growth in the in-vitro screening and area under disease progress curve (AUDPC) in the <em>in-planta</em> screening, and species identities and GenBank accession numbers of 147 identified bacterial strains.</p></li></ul><p></p>
| Main Authors: | , |
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| Format: | Dataset biblioteca |
| Published: |
2019
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| Subjects: | Crop and pasture production, Plant pathology, Plant biology not elsewhere classified, Buxus sempervirens, blight, Calonectria, fungi, United States, landscapes, industry, irrigation systems, biological control, Pseudomonas protegens, mycelium, in vitro studies, secondary metabolites, mechanism of action, Cylindrocladium, ornamental trees, fungal diseases of plants, plant pathogenic fungi, nursery crops, biopesticides, microbial pesticides, biological control agents, microbial toxins, fungal antagonists, toxigenic strains, emerging diseases, |
| Online Access: | https://figshare.com/articles/dataset/Data_from_Biological_control_of_boxwood_blight_by_Pseudomonas_protegens_recovered_from_recycling_irrigation_systems/24853014 |
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| Summary: | <p>Boxwood (<em>Buxus</em> spp.) blight caused by <em>Calonectria pseudonaviculata</em> (<em>Cps</em>) is an emerging fungal disease in the United States. It has caused significant loss to the ornamental nursery and landscape industries. In this study, 1547 bacterial strains recovered from recycling irrigation systems were evaluated <em>in vitro</em> and <em>in planta</em> for their biological control efficacy against <em>Cps</em>. Three most potent strains 13A3, 14D5, and 34B6 identified all belonged to <em>Pseudomonas protegens</em>. Strains 13A3 and 14D5 reduced <em>Cps</em> mycelial growth by 95 to 100% in two sets of <em>in vitro</em> experiments including a 48-well plate assay and a dual culture assay. Strain 34B6 also reduced <em>Cps</em> growth by 93% in the 48-well plate assay and 66 to 72% in dual culture plates. <em>In planta</em>, strain 14D5 reduced <em>Cps</em> infection by more than 50%, while strains 13D3 and 34B6 by 40 to 46%. <em>Pseudomonas protegens</em> strains produced antifungal secondary metabolites that reduced <em>Cps</em> growth and infection. Other possible modes of action against boxwood blight are discussed. </p><div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Supplementary Table S1.</p> <p>File Name: Web Page, url: <a href="https://www.sciencedirect.com/science/article/pii/S1049964418300434?via=ihub#s0095">https://www.sciencedirect.com/science/article/pii/S1049964418300434?via=ihub#s0095</a> </p><p>Bacterial strains, the reduction of <em>Cps</em> growth in the in-vitro screening and area under disease progress curve (AUDPC) in the <em>in-planta</em> screening, and species identities and GenBank accession numbers of 147 identified bacterial strains.</p></li></ul><p></p> |
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