Selecting a core set of nuclear SNP markers for molecular characterization of Arabica coffee (Coffea arabica L.) genetic resources
Cofee is one of the most economically important agricultural commodities in the world. Labeling accuracy and conservation efciency are essential for cofee germplasm management and for the exchange and utilization in breeding new varieties. However, due to its homogenous genetic background, accurate identifcation of Cofea arabica germplasm has not been fully achieved. Specifcally, data comparison across diferent laboratories and genotyping platforms has not been available. Here, we report the screening of 672 candidate SNPs using Nano-Fluidic Array genotyping. Based on call rate, Minor Allele Frequency and Linkage Disequilibrium, a set of 96 SNPs were selected for genotyping C. arabica. This validated panel is suitable for use in cofee germplasm conservation and crop improvement, including varietal identifcation, seeds and nursery accreditation, and cofee bean authentication.
| Main Authors: | , , , , , |
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| Format: | Texto biblioteca |
| Language: | eng |
| Subjects: | Coffea arabica, Recursos de germoplasma vegetal, Marcadores genéticos, Agricultura tropical, |
| Online Access: | https://link.springer.com/article/10.1007/s12686-021-01201-y |
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| Summary: | Cofee is one of the most economically important agricultural commodities in the world. Labeling accuracy and conservation efciency are essential for cofee germplasm management and for the exchange and utilization in breeding new varieties. However, due to its homogenous genetic background, accurate identifcation of Cofea arabica germplasm has not been fully achieved. Specifcally, data comparison across diferent laboratories and genotyping platforms has not been available. Here, we report the screening of 672 candidate SNPs using Nano-Fluidic Array genotyping. Based on call rate, Minor Allele Frequency and Linkage Disequilibrium, a set of 96 SNPs were selected for genotyping C. arabica. This validated panel is suitable for use in cofee germplasm conservation and crop improvement, including varietal identifcation, seeds and nursery accreditation, and cofee bean authentication. |
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