Propagation of arabica coffee by micro cuttings
Results from experiments conducted to optimize a protocol for micropropagation of coffee (Coffea arabica) using nodal explants are presented. Multiple shoot production from aseptic nodal segments was recorded in 44-75 percent of the explants. Rooting in net pots in a polytunnel filled with moist sand was obtained from 95-98 percent of the shoots. From each nodal explant, 48-60 shoots could be obtained through this protocol, suggesting an economically viable protocol for the production of elite planting materialsthrough tissue culture. Also, organogenesis was recorded for the first time from the green callus produced from the lower end of nodal explant in contact with the medium in 21-34 percent of the cultures.
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| Main Authors: | , , |
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| Format: | biblioteca |
| Published: |
2000
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| Subjects: | COFFEA ARABICA, CALLO, CAFE, MEDIO DE CULTIVO, CORTE, EXPLANTES, CULTIVO IN VITRO, MICROPROPAGACION, ORGANOGENESIS, MATERIALES DE PROPAGACION, RAICES, TALLOS, CULTIVO DE TEJIDOS, |
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| Summary: | Results from experiments conducted to optimize a protocol for micropropagation of coffee (Coffea arabica) using nodal explants are presented. Multiple shoot production from aseptic nodal segments was recorded in 44-75 percent of the explants. Rooting in net pots in a polytunnel filled with moist sand was obtained from 95-98 percent of the shoots. From each nodal explant, 48-60 shoots could be obtained through this protocol, suggesting an economically viable protocol for the production of elite planting materialsthrough tissue culture. Also, organogenesis was recorded for the first time from the green callus produced from the lower end of nodal explant in contact with the medium in 21-34 percent of the cultures. |
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