Extracellular proteins as markers of embryogenic potential in Coffea spp. suspension cultures
Extracellular proteins EP3 and PE32 in Coffea canephora var. Robusta and Coffea arabica cv. Catimor cell suspension cultures were studied. The embryogenic process was immunochemically characterized by polyclonal antibodies of both proteins. The behaviour of extracellular proteins proved the embryogenic character of the culture. The use of these proteins as molecular markers is suggested for coffee somatic embryogenesis.
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| Main Authors: | , , |
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| Format: | biblioteca |
| Published: |
2000
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| Subjects: | COFFEA ARABICA, COFFEA CANEPHORA, CAFE, CULTIVO DE CELULAS, PROTEINAS VEGETALES, EMBRIOGENESIS SOMATICA, INMUNOLOGIA, |
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| Summary: | Extracellular proteins EP3 and PE32 in Coffea canephora var. Robusta and Coffea arabica cv. Catimor cell suspension cultures were studied. The embryogenic process was immunochemically characterized by polyclonal antibodies of both proteins. The behaviour of extracellular proteins proved the embryogenic character of the culture. The use of these proteins as molecular markers is suggested for coffee somatic embryogenesis. |
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