Desarrollo in vitro de la broca del café, Hypothenemus hampei (Coleoptera: Scolytidae), en una dieta meridica liofilizada
Freeze-drying is a useful alternative for preparation and conservation of the meridic diet used for laboratory rearing of Hypothenemus hampei. This process allows preparation of diets for insect rearing without altering sensory and physicochemical characteristics of the final product, substantially improving traditional stove drying. Humidities of the freeze-dried diet and the stove dried diet were evaluated during 20 days of storage at 27°2°C. Decrease in humidity in the freeze-dried diet was 10 percent and in the stove dried diet was 34 percent. The freeze-dried diet remained stable when stored during 20 days at -15°C (frozen) and at 4°C (refrigerated). Forty days after infestation, the average of biological stages in the freeze-dried diet was 48.3 stages/borer, significantly higher (Tukey 5 percent) than that found in the stove dried diet (15.3). Development of the coffee berry borer from egg to adult was completed in 24 days with 94 percent efficiency. The use of a freeze-dried diet will facilitate the study of protein fractions with toxic activity against digestive enzymes of various insects, which could further be used in plant breeding through molecular biology techniques.
| Main Authors: | , , |
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| Format: | biblioteca |
| Published: |
2000
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| Subjects: | COFFEA, DESARROLLO BIOLOGICO, HYPOTHENEMUS HAMPEI, LIOFILIZACION, PLAGAS DE PLANTAS, |
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| Summary: | Freeze-drying is a useful alternative for preparation and conservation of the meridic diet used for laboratory rearing of Hypothenemus hampei. This process allows preparation of diets for insect rearing without altering sensory and physicochemical characteristics of the final product, substantially improving traditional stove drying. Humidities of the freeze-dried diet and the stove dried diet were evaluated during 20 days of storage at 27°2°C. Decrease in humidity in the freeze-dried diet was 10 percent and in the stove dried diet was 34 percent. The freeze-dried diet remained stable when stored during 20 days at -15°C (frozen) and at 4°C (refrigerated). Forty days after infestation, the average of biological stages in the freeze-dried diet was 48.3 stages/borer, significantly higher (Tukey 5 percent) than that found in the stove dried diet (15.3). Development of the coffee berry borer from egg to adult was completed in 24 days with 94 percent efficiency. The use of a freeze-dried diet will facilitate the study of protein fractions with toxic activity against digestive enzymes of various insects, which could further be used in plant breeding through molecular biology techniques. |
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