Somatic embryogenesis from three coffee cultivars: 'Rubi', 'Catuai Vermelho 81', and 'IAPAR 59'
Leaf explants of Coffea arabica cultivars Rubi, Catua{DD} Vermelho 81 and IAPAR 59 were in vitro cultured on MS medium with 4 micro M of picloram for 8-12 weeks in the dark at 27 plus or minus 2 deg C (standard temperature). Rubi and Catuaí Vermelho 81 explants with callus were transferred to an SP basal medium plus 0, 12, 24, and 48 micro M benzyladenine, whereas for IAPAR 59, only 48 micro M benzyladenine was added. After 8 weeks for Rubi and Catua{DD} Vermelho 81 and after 12 weeks for IAPAR 59, all cultivars showed indirect embryogenesis. Embryogenic material was transferred to an SP basal medium without benzyladenine, in light (16-hour photoperiod) and standard temperature. In a second experiment, leaf explants belonging to all 3 cultivars were inoculated in petri dishes with SP basal medium plus 0, 2.5 and 5 micro M benzyladenine, in the dark at standard temperature. After 8 weeks, only IAPAR 59 produced direct embryos. These were transferred to SP basal medium. Approximately 30 percent ofthe embryos developed abnormalities. Normal embryos were successfully transferred to soil.
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| Format: | biblioteca |
| Published: |
2004
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| Subjects: | COFFEA, COFFEA ARABICA, RUBIACEAE, RUBIALES, DICOTILEDONEAS, ANGIOSPERMAS, SPERMATOPHYTA, TRASTORNOS FUNCIONALES, 6-BENZILADENINA, MEDIO DE CULTIVO, CULTIVO IN VITRO, REGENERACION IN VITRO, HOJAS, PICLORAN, SUSTANCIAS DE CRECIMIENTO VEGETAL, EMBRIOGENESIS SOMATICA, EMBRION SOMATICO, CULTIVO DE TEJIDOS, |
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| Summary: | Leaf explants of Coffea arabica cultivars Rubi, Catua{DD} Vermelho 81 and IAPAR 59 were in vitro cultured on MS medium with 4 micro M of picloram for 8-12 weeks in the dark at 27 plus or minus 2 deg C (standard temperature). Rubi and Catuaí Vermelho 81 explants with callus were transferred to an SP basal medium plus 0, 12, 24, and 48 micro M benzyladenine, whereas for IAPAR 59, only 48 micro M benzyladenine was added. After 8 weeks for Rubi and Catua{DD} Vermelho 81 and after 12 weeks for IAPAR 59, all cultivars showed indirect embryogenesis. Embryogenic material was transferred to an SP basal medium without benzyladenine, in light (16-hour photoperiod) and standard temperature. In a second experiment, leaf explants belonging to all 3 cultivars were inoculated in petri dishes with SP basal medium plus 0, 2.5 and 5 micro M benzyladenine, in the dark at standard temperature. After 8 weeks, only IAPAR 59 produced direct embryos. These were transferred to SP basal medium. Approximately 30 percent ofthe embryos developed abnormalities. Normal embryos were successfully transferred to soil. |
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