Characterization of a partial sequence of the P-subunit of the glycine decarboxylase gene from four coffea genotypes
A 427-bp fragment of DNA isolated from leaf tissue of four coffee genotypes (Coffea arabica cv. Caturra, C. canephora, C. congensis and C. eugenioides) was amplified by PCR using heterologous primers synthesized following the nucleotide sequence encoding a part of P-subunit of glycine decarboxylase from pea (Pisum sativum). Amplified fragments were cloned and their nucleotide sequence was determined. Sequence analysis showed 79 percent homology between the gene sequence of the P-subunit of glycine decarboxylase from P. sativum and the four Coffea genotypes. The amino acid sequence of cloned fragments showed 81 percent homology between P. sativum and the four Coffea genotypes, whereas between Flaveria pringlei and Coffea such homology was 85 percent. Comparison of nucleotide sequences for the P-subunit gene from the four Coffea genotypes showed 98-100 percent and 99-100 percent homology at the amino acid level, except for a substitution of glutamine by glutamic acid (position 59) in C. canephora and C. congensis.
| Main Authors: | , , |
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| Format: | biblioteca |
| Published: |
2001
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| Subjects: | COFFEA, COFFEA ARABICA, COFFEA CANEPHORA, COFFEA CONGENSIS, COFFEA EUGENIOIDES, SECUENCIAS DE AMINOACIDOS, CAFE, GENES, GENOTIPOS, ACIDO GLUTAMICO, GLUTAMINA, HOJAS, SECUENCIA NUCLEOTIDICA, |
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| Summary: | A 427-bp fragment of DNA isolated from leaf tissue of four coffee genotypes (Coffea arabica cv. Caturra, C. canephora, C. congensis and C. eugenioides) was amplified by PCR using heterologous primers synthesized following the nucleotide sequence encoding a part of P-subunit of glycine decarboxylase from pea (Pisum sativum). Amplified fragments were cloned and their nucleotide sequence was determined. Sequence analysis showed 79 percent homology between the gene sequence of the P-subunit of glycine decarboxylase from P. sativum and the four Coffea genotypes. The amino acid sequence of cloned fragments showed 81 percent homology between P. sativum and the four Coffea genotypes, whereas between Flaveria pringlei and Coffea such homology was 85 percent. Comparison of nucleotide sequences for the P-subunit gene from the four Coffea genotypes showed 98-100 percent and 99-100 percent homology at the amino acid level, except for a substitution of glutamine by glutamic acid (position 59) in C. canephora and C. congensis. |
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