Picomolar concentrations of salicylates induce cellular growth and enhance somatic embryogenesis in Coffea arabica tissue culture
Embryogenic cell suspension cultures of C. arabica cv. Caturra Rojo were treated with salicylic acid (SA). Two concentrations, 10-12 and 10-10 M, had a significant effect on the growth rate of the cell cultures compared to the control, and this effect was concentration dependent. These two SA concentrations also had a dramatic effect on both the number of somatic embryos and quality, in terms of embryo size and development. In general, the use of SA had a positive effect on cellular growth and somatic embryogenesis, causing a two-fold increase in both processes. The increase in the number of somatic embryos could be a reflection of an increase in the number of embryogenic cells induced with SA treatment.
Main Authors: | , , , |
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Format: | biblioteca |
Published: |
2001
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Subjects: | COFFEA ARABICA, CULTIVO DE CELULAS, TECNICAS DE CULTIVO, CAFE, CULTIVO IN VITRO, EMBRIOGENESIS SOMATICA, CULTIVO DE TEJIDOS, |
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Summary: | Embryogenic cell suspension cultures of C. arabica cv. Caturra Rojo were treated with salicylic acid (SA). Two concentrations, 10-12 and 10-10 M, had a significant effect on the growth rate of the cell cultures compared to the control, and this effect was concentration dependent. These two SA concentrations also had a dramatic effect on both the number of somatic embryos and quality, in terms of embryo size and development. In general, the use of SA had a positive effect on cellular growth and somatic embryogenesis, causing a two-fold increase in both processes. The increase in the number of somatic embryos could be a reflection of an increase in the number of embryogenic cells induced with SA treatment. |
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